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Category: TopoGEN News

Cell Context DNA Repair Kits Now Available

Cell Context DNA Repair Kits Now Available

Are you working on DNA-damage and repair?  Would you be interested in a pre-validated cell-context system to analyze HR or NHEJ by single cell imaging or quantifying DNA repair by simple fluorescence? TopoGEN, Inc. has developed a suite of cell lines that will allow you to study specific DNA repair pathways in living cells. Our systems report specific Homologous Recombination (HR) well as Non-Homologous End Joining (NHEJ) pathways.  These engineered cell lines are truly novel, giving unprecedented insight into HR…

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Topoisomerase II Drug Screening and Xanthocidin

Topoisomerase II Drug Screening and Xanthocidin

These researchers have cleverly identified a way to synthesize derivatives of an unstable compound group with potential as Topoisomerase IIa inhibitors. Topo II and Drug Screening kits for the analysis of enzyme reactivity and DNA cleavage were provided by TopoGEN. Article Source: The Pharmaceutical Society of Japan Biological and Pharmaceutical Bulletin Xanthocidin Derivatives as Topoisomerase IIα Enzymatic Inhibitors Abstract Few studies have examined xanthocidin, a biotic isolated from Streptomyces xanthocidicus in 1966, because its supply is limited. Based on its…

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Topoisomerase II Targeting and Resveratrol

Topoisomerase II Targeting and Resveratrol

In this paper the authors use a decatenation assay to identify some new topoisomerase II inhibitors from resveratrol. kDNA substrate was provided by TopoGEN. Article Source: Journal of Agricultural and Food Chemistry Topoisomerase II-Targeting Properties of a Grapevine-Shoot Extract and Resveratrol Oligomers Abstract Grapevine-shoot extracts (GSE), containing trans-resveratrol and resveratrol oligomers, are commercially available as food supplements. Considering the topoisomerase-targeting properties of trans-resveratrol, the question of whether GSE affect these enzymes, thereby potentially causing DNA damage, was addressed. In a…

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Findings on the Dissasemblage of Particular Nuclear Subdomain in Tumor Cells

Findings on the Dissasemblage of Particular Nuclear Subdomain in Tumor Cells

The authors discuss the particulars of the perinucluear compartment in DNA and its effects on the DNA integrity of a tumor rich environment. Human Topo I Antibody provided by TopoGEN. Article Source: The Journal of Biological Chemistry The Perinucleolar Compartment Is Directly Associated with DNA Abstract The perinucleolar compartment (PNC) is a nuclear subdomain that is unique to tumor cells, and the percentage of cells in a population containing PNCs (PNC prevalence) indicates the level of malignancy of that population….

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ICE Bioassay: Use and Application

ICE Bioassay: Use and Application

This short video describes how our in vivo Link kits for Topo I and Topo II allow the investigator to observe how drug action inside a tumor cell. Topo I in vivo Link Kit and Topo II in vivo Link Kit available from TopoGEN. Topo I in vivo Link Kit and Topo II in vivo Link Kit available from TopoGEN.

New: Updated Kit Protocols…Now Available for Download!

New: Updated Kit Protocols…Now Available for Download!

We have updated the protocols of our most popular Topoisomerase Assay and Drug Screening Kits – better graphics, an FAQ section, and visual layouts that are easier on the eye.  Whats more is that these protocols are now available for download here!  Check back as we upload more protocols in the coming weeks.  The following new protocols are available on the Product Page for each kit under “Related Documents:” Human Topoisomerase II Assay Kit TG1001 Topoisomerase I Assay Kit TG1015…

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Topo I Enzyme Provided for Autoimmunity Study

Topo I Enzyme Provided for Autoimmunity Study

Immunization with DNA topoisomerase I and Freund’s complete adjuvant induces skin and lung fibrosis and autoimmunity via interleukin-6 signaling. Abstract OBJECTIVE: The presence of anti-DNA topoisomerase I (anti-topo I) antibody correlates positively with disease severity in patients with systemic sclerosis (SSc). However, the role of induction of anti-topo I antibody production and its potential contribution to the pathogenesis of SSc remain unclear. The aim of this study was to examine the role of anti-topo I antibody in the pathogenesis of…

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New Topoisomerase II Inhibitor Study

New Topoisomerase II Inhibitor Study

This Merck study was published in Cell and showcases the use of our E. coli DNA Gyrase and Topo IV assay protocols in identifying a new class of Topo II inhibitor.  The following type II topoisomerases were provided for the study by TopoGEN: E. coli DNA Gyrase, Topoisomerase IV.  The following plasmids were also provided by TopoGEN: pBR322, kDNA. Article Source: Cell Website