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Category: TopoGEN News

Topoisomerase II Targeting and Resveratrol

Topoisomerase II Targeting and Resveratrol

In this paper the authors use a decatenation assay to identify some new topoisomerase II inhibitors from resveratrol. kDNA substrate was provided by TopoGEN. Article Source: Journal of Agricultural and Food Chemistry Topoisomerase II-Targeting Properties of a Grapevine-Shoot Extract and Resveratrol Oligomers Abstract Grapevine-shoot extracts (GSE), containing trans-resveratrol and resveratrol oligomers, are commercially available as food supplements. Considering the topoisomerase-targeting properties of trans-resveratrol, the question of whether GSE affect these enzymes, thereby potentially causing DNA damage, was addressed. In a…

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Findings on the Dissasemblage of Particular Nuclear Subdomain in Tumor Cells

Findings on the Dissasemblage of Particular Nuclear Subdomain in Tumor Cells

The authors discuss the particulars of the perinucluear compartment in DNA and its effects on the DNA integrity of a tumor rich environment. Human Topo I Antibody provided by TopoGEN. Article Source: The Journal of Biological Chemistry The Perinucleolar Compartment Is Directly Associated with DNA Abstract The perinucleolar compartment (PNC) is a nuclear subdomain that is unique to tumor cells, and the percentage of cells in a population containing PNCs (PNC prevalence) indicates the level of malignancy of that population….

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ICE Bioassay: Use and Application

ICE Bioassay: Use and Application

This short video describes how our in vivo Link kits for Topo I and Topo II allow the investigator to observe how drug action inside a tumor cell. Topo I in vivo Link Kit and Topo II in vivo Link Kit available from TopoGEN. Topo I in vivo Link Kit and Topo II in vivo Link Kit available from TopoGEN.

New: Updated Kit Protocols…Now Available for Download!

New: Updated Kit Protocols…Now Available for Download!

We have updated the protocols of our most popular Topoisomerase Assay and Drug Screening Kits – better graphics, an FAQ section, and visual layouts that are easier on the eye.  Whats more is that these protocols are now available for download here!  Check back as we upload more protocols in the coming weeks.  The following new protocols are available on the Product Page for each kit under “Related Documents:” Human Topoisomerase II Assay Kit TG1001 Topoisomerase I Assay Kit TG1015…

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Topo I Enzyme Provided for Autoimmunity Study

Topo I Enzyme Provided for Autoimmunity Study

Immunization with DNA topoisomerase I and Freund’s complete adjuvant induces skin and lung fibrosis and autoimmunity via interleukin-6 signaling. Abstract OBJECTIVE: The presence of anti-DNA topoisomerase I (anti-topo I) antibody correlates positively with disease severity in patients with systemic sclerosis (SSc). However, the role of induction of anti-topo I antibody production and its potential contribution to the pathogenesis of SSc remain unclear. The aim of this study was to examine the role of anti-topo I antibody in the pathogenesis of…

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New Topoisomerase II Inhibitor Study

New Topoisomerase II Inhibitor Study

This Merck study was published in Cell and showcases the use of our E. coli DNA Gyrase and Topo IV assay protocols in identifying a new class of Topo II inhibitor.  The following type II topoisomerases were provided for the study by TopoGEN: E. coli DNA Gyrase, Topoisomerase IV.  The following plasmids were also provided by TopoGEN: pBR322, kDNA. Article Source: Cell Website

Topoisomerase I and DNA Relaxation

Topoisomerase I and DNA Relaxation

Par-4 Binds to Topoisomerase I and Attenuates Its DNA Relaxation Activity The authors of this paper have identified a tumor suppressor protein that regulates Topo I, and in so doing provide a nice review of the mechanistic nature of DNA and cancer cell metabolism.  Human Topo I and polyclonal antibodies were provided by TopoGEN. Article Source: Cancer Research Website

Custom Drug Screening Services

Custom Drug Screening Services

In addition to manufacturing, TopoGEN has quietly developed into a CRO powerhouse in recent years.  Utilizing our own regimes and expertise that comes with 30+ years of experience, we tailor-build a custom screening solution for your novel anti-cancer studies.  Specific targets include eukaryotic topoisomerases (topo I, topo II, topo III), prokaryotic topoisomerases (gyrase, topo IV) in E. Coli and S. Aureus, and recently we added DNA methyltransferase targets.  We are also developing a solid phase assay for topo I (provisional…

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Novel Gold Compounds Potential Anti-cancer Therapies

Novel Gold Compounds Potential Anti-cancer Therapies

TopoGEN is partnering with a prominent analytical chemist based in South Africa on the development of innovative anti-topo I and II targeting drugs based on synthetic structures containing gold.  Evidence demonstrates the combination of poisoning and catalytic inhibition of the DNA topoisomerase pathways.  Initial screens performed by NCI demonstrated efficacy in cancer and subsequent confirmation by TopoGEN is showing topoisomerase targeting.   Further studies are pending SBIR phase I review and the acquisition of patents filed in the U.S. and abroad. …

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