Relaxed pRYG DNA

SKU	Product	Size	Price	Buy
TG2025-1	Relaxed pRYG DNA	10 ug	$149.00

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Human Topoisomerase II pRYG DNA

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pRYG is a pUC19 derivative in which the 206 bp SphI-NarI fragment of pUC19 was replaced with a 245 bp SphI-AccI fragment upstream of the human adult beta-globin gene promoter. The 245 bp fragment contains a 54 bp alternating repeat of purine/pyrimidine sequence that constitutes a strong eukaryotic topo II cleavage site.  Cleavages within the repeat are readily detected in the absence of topo II inhibitors such as m-AMSA or VM-26.  The sequence of the plasmid is given in Table 2 of Spitzner et al.


Reference:
For detailed description of pRYG, its reactivity with Topo II and the sequence, see Spitzner et al., Nuc. Acid. Res. 18: 1-11 (1990)

Shipping and Storage Instructions:
The marker is shipped at ambient temperature and should be stored a 4° C.