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DNA Substrates
Topoisomerase enzymes are sensitive to DNA quality (for example, heavy metal contamination) and not all DNA preps will support activity. All of our DNA substrates are screened to support topoisomerase (and gyrase) activity. When you do screening assays for DNA cleavages, you must have DNA with minimal contamination of nicked forms. Fortunately, TopoGEN controls plasmid and kDNA stocks for nicked contaminants, so you don’t have to worry about wasting expensive enzymes on inferior DNA substrate.
Kinetoplast DNA (kDNA)
Kinetoplast DNA (catenated): the ideal topoisomerase II substrate.
| SKU | Product | Size | Price | Buy |
|---|---|---|---|---|
| TG2013-1 | Kinetoplast DNA (kDNA) | 25ug | $199.00 |  |
| TG2013-2 | Kinetoplast DNA (kDNA) | 50ug | $299.00 | |
| TG2013-3 | Kinetoplast DNA (kDNA) | 250ug | $699.00 | |
pRYG DNA
pRYG DNA is a plasmid containing multiple clusters of topoisomerase II cleavage sites.
| SKU | Product | Size | Price | Buy |
|---|---|---|---|---|
| TG2023-2 | pRYG DNA | 20 ug | $199.00 | |
pHOT-1 DNA
pHOT-1 DNA is a high affinity Topoisomerase I cleavage substrate.
| SKU | Product | Size | Price | Buy |
|---|---|---|---|---|
| TG2030-2 | pHOT-1 DNA | 20 ug | $199.00 | |
Supercoiled pBR322 DNA (modified)
Ideal plasmid for assaying any topoisomerase.
| SKU | Product | Size | Price | Buy |
|---|---|---|---|---|
| TG2036-1 | Supercoiled pBR322 DNA (modified) | 50 ug | $229.00 | |
| TG2036-2 | Supercoiled pBR322 DNA (modified) | 250 ug | $559.00 | |
Relaxed pBR322 DNA
Relaxed pBR322 DNA - an ideal plasmid for assaying supercoiling by DNA gyrase.
| SKU | Product | Size | Price | Buy |
|---|---|---|---|---|
| TG2037-1 | Relaxed pBR322 DNA | 50 ug | $249.00 | |
| TG2037-2 | Relaxed pBR322 DNA | 250 ug | $599.00 | |