Fig. 1

Figure 1. Topo II and DNA gyrase reaction products with kDNA substrate.

The Data shown below are based upon the following concept

Shown below is an idealized view of kDNA reactivity with eukaryotic topo II and prokaryotic DNA gyrase. From Left to right:

kDNA Marker: Most of the kDNA will be high MW catenanes that fail to enter the gel
Topo II Lest activity: Low enzyme and high enzyme activity levels are shown. At low enzyme activity, some kDNA may remain in the wells; at higher levels, all of the kDNA is released as either OC nicked monomers or relaxed circularized monomer.
Linear kDNA Marker: Produced by incubating with a restriction enzyme that cuts kDNA monomers once.
Decatenated kDNA Marker: Shows relative positions of OC nicked and circular monomers
DNA gyrase products: Since gyrase is a topo II, it will decatenate kDNA; however, it will then supercoil the circular monomers.