Gyrase 2000G

Bacterial (E. coli) DNA Gyrase

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Description:

Highly purified E. coli gyrase is being offered as a holoenzyme (contains both A,B subunits). This is an excellent reagent for supercoiling plasmids in vitro or for novel drug screens.

Quality Control Tests:

Nuclease contamination: assayed by testing for linear KDNA and linear plasmid DNA formation. Incubation of 1 µg of catenated KDNA or supercoiled pUC19 DNA for 4 hrs. at 37 C (under gyrase assay conditions and with or without ATP).

Assay Conditions:

Supercoiling assays are carried out using relaxed pBR-322 DNA under conditions specified in the protocol sheets provided with the product. We also perform assays using kDNA as substrate. For details on this decatenation/supercoiling assay go here. We also have some technical literature on assays: GO HERE. One unit of gyrase activity will supercoil 0.25 ug pBR-322 DNA in 30 min at 37 C.

A 5X assay buffer and dilution buffer is included with the enzyme.

Storage and Shipping Conditions:

The enzyme is shipped on dry ice and should be stored at -70 C. We also recommend that the enzyme be aliquoted after the first thaw (repeated rounds of freeze/thaw may cause loss of activity); the enzyme activity is stable for 1-3 days on ice.

Catalog Number	Description	Size
2000G-1	DNA Gyrase	100 units
2000G-3	DNA Gyrase	500 units
2000G-5	DNA Gyrase	1000 units
2000G-7	DNA Gyrase	2000 units

Please enquire about bulk sales of DNA gyrase greater than 2000 Units. We offer discounts on bulk Gyrase above 2000 units. Contact us at topogen@aol.com for a quote.

If you are working with Gyrase, the following products will be of interest

Catalog Number

Description

Size

2000G1KIT

DNA Gyrase+relaxed DNA + Rxn Buffer

100 units

2013-1

kDNA substrate

25 ug

.