Doing some quantitative topo assays?  Want a surefire way to quantify your gel data?  We have a Gel Documentation system that will knock your socks off! READ MORE HERE or go to the PDF link below to get detailed information about GeneFlash!

In depth description of GeneFlash Applications with TopoGEN Assays (544 kb)

Mutant Human Topo I Enzyme 2005H-Y723F

Recombinant Human Topo I Enzyme Overexpressed in baculovirus 2005H-RC1.  We can supply LARGE quantities of topo I for physical studies.

Topo I Western Blot Marker 2011-4

Topo I MW 
Marker on 
Western blot

Now available.... Tdp1

T o TopoGEN is the first to provide highly purified Tdp1 (Tyrosyl DNA Phodphodiesterase) and related reagents. Tdp1 is the enzyme that removes the covalently bound topo from cleavage complexes. Our Tdp1 has excellent catalytic activity and is ideal for drug screening. Related reagents are a Tdp1 assay kit (for measuring Tdp1 activity in cells and tumors and for drug discovery) and anti-Tdp1 antibody.  Tdp1 is showing excellent potential as a new combinatorial drug target, along with topo I. Some recent papers are now out on this exciting new target (go here).

Schematic of Tdp1 activity.  Tdp1 releases topo I from DNA as shown in the outline above.  
Covalent complexes are thought to be repaired by this mechanism.

Topo I overexpressed in Baculovirus
and analyzed on SDS-PAGE

New Polyclonal Antibodies to Topo II beta (2010-3) and Topo III beta (2010-4)

We offer custom screening on a contract basis. Let us test your compounds for topo targeting activity.

Print our catalog in acrobat format (PDF).

TopoGEN New Products

Technical Questions?
Email support@topogen.com

MAILING ADDRESS
TopoGEN, Inc.
108 Aces Alley
Port Orange, FL, USA 32128
info@topogen.com
Tel  614-451-5810
Fax  614-559-3932

Want to quantify your topo gel assay data or topo cleavage data?
GO HERE for more information.

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Updated December 26, 2006