Description:
The marker set for topo II is used to unambiguously locate the position of the 170 kDa topo II in Western Blots. The human 170 kDa is present in SDS-PAGE loading buffer. Note that human topo II may break down spontaneously with prolonged storage or with repeated freezing and thawing cycles. TopoGEN, Inc. stipulates that the marker be 90% undegraded at the time of shipment.
Quality Control:
1. The presence of the 170 band is confirmed by Western blotting; less than 5-10% degradation of the 170 band is detected.
2. There is no 180 kDa topo II in this preparation.
3. Loading 10 ul of extract is sufficient for use as a marker in Western blotting.*
Shipping/Storage Conditions:
The polypeptide is shipped on dry ice and is supplied in SDS-PAGE gel loading buffer. The tube should be divided into aliquots after thawing; store the aliquots at -70oC.
*By special order, TopoGEN can supply enough of this marker to visualize the p170 band by commasie blue staining; please enquire about Catalog Number 2011-3C.
Material safety data:
THIS PRODUCT IS NOT LICENSED OR APPROVED FOR ADMINISTRATION TO HUMANS OR TO ANIMALS OTHER THAN EXPERIMENTAL ANIMALS.
Cat.#:
Purified p170 kDa (sufficient for 20 gel loads)
Description:
Data Sheet for this product
We offer custom screening on a contract basis. Let us test your compounds for topo targeting activity. 
Print our catalog in acrobat format (PDF).
Technical Questions?
Email support@topogen.com
MAILING ADDRESS
TopoGEN, Inc.
108 Aces Alley
Port Orange, FL, USA 32128
info@topogen.com
Tel 614-451-5810
Fax 614-559-3932
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![Topo II [p170] Marker](../assets/images/autogen/Topo_II__p170__Marker_Hs3.gif "Topo II [p170] Marker"){kind=small} |
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Want to quantify your topo gel assay data or topo cleavage data?
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Updated December 26, 2006
