Panel B,C

Gel Origin

OC DNA+ CCC relaxed DNA

CCC Supercoiled DNA

Panel B: Typical Non-EB containing gel showing the separation of the different plasmid DNA forms. Resolution of supercoiled DNA from nicked (open cirucular, OC) and relaxed (covalently closed circular, ccc) DNA is excellent. NOTE: Relaxed CCC DNA and nicked OC DNA may resolve poorly (if at all) in this system. These types of gels are ideal if you want to quickly test for relaxation activity. They are less than ideal for detecting OC nicked DNA products that arise from topo I action on DNA in the presence of drugs or poisons like camptothecin.

Linear DNA

Gel Origin

OC DNA

Topo relaxation products: Topoisomers (relaxed DNA forms that differ in linking number )

CCC Supercoiled DNA

Panel C: Typical Non-EB containing gel separation of OC Nicked DNA, ccc Supercoiled DNA and and a gaussian distribution of topoisomers. The formation of these topoisomerase are diagnostic for topo I or II activity. For this reason, these gels are ideal for activity measurements.

In depth description of GeneFlash Applications with TopoGEN Assays (544 kb) and some actual gel data.

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