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Name

DNA Intercalator/Unwinding Kit 50 Experiments

Price

$349.00

SKU/Part Number

1025

Extended Description

The purpose of this kit is to determine if a compound induces DNA unwinding characteristic of DNA intercalators. Sufficient materials are provided for at least 50 independent determinations.

NOTE: enzyme is not included with this kit.

Kit Contents:
-Substrate DNA: pHOT1 DNA
-Relaxed DNA Marker: 0.05 ug/ul in 1x gel loading buffer
-10 x Topo I assay buffer, Cat#4010 (750 µl)
-10x Gel Loading buffer, Cat#4080 (750 ul)
-10% SDS, Cat# 4060 (750 ul)
-mAMSA Stock, Lyophilized (control intercalator)
-Detailed Instruction Manual

The following materials and reagents are not supplied with this kit but will be required:
-Agarose and related electrophoretic supplies and equipment
-Transilluminator and photodocumentation system
-DNA extraction and purification related reagents: Proteinase K, Choloroform: Isolamyl alcohol (24:1)

Shipping and Recommended Storage Conditions:
This kit is shipped at ambient temperature. Store at 4 C.

Related Products: p170 Human Topo II (Cat. No. 2000H), Human Topo I (Cat. No. 2005H), Camptothecin (Cat. No. 4110), Doxorubicin (Cat. No. 4120), Daunomycin (Cat. No. 4130), Etoposide (Cat. No. 4140), m-AMSA (Cat. No. 4150), Genistein (Cat. No. 4160), Ellipticine (Cat. No. 4170)

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