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Name
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DNA Intercalator/Unwinding Kit 50 Experiments
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Price
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$349.00
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SKU/Part Number
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1025
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Extended Description
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The purpose of this kit is to determine if a compound induces DNA unwinding characteristic of DNA intercalators. Sufficient materials are provided for at least 50 independent determinations.
NOTE: enzyme is not included with this kit.
Kit Contents: -Substrate DNA: pHOT1 DNA -Relaxed DNA Marker: 0.05 ug/ul in 1x gel loading buffer -10 x Topo I assay buffer, Cat#4010 (750 µl) -10x Gel Loading buffer, Cat#4080 (750 ul) -10% SDS, Cat# 4060 (750 ul) -mAMSA Stock, Lyophilized (control intercalator) -Detailed Instruction Manual
The following materials and reagents are not supplied with this kit but will be required: -Agarose and related electrophoretic supplies and equipment -Transilluminator and photodocumentation system -DNA extraction and purification related reagents: Proteinase K, Choloroform: Isolamyl alcohol (24:1)
Shipping and Recommended Storage Conditions: This kit is shipped at ambient temperature. Store at 4 C.
Related Products: p170 Human Topo II (Cat. No. 2000H), Human Topo I (Cat. No. 2005H), Camptothecin (Cat. No. 4110), Doxorubicin (Cat. No. 4120), Daunomycin (Cat. No. 4130), Etoposide (Cat. No. 4140), m-AMSA (Cat. No. 4150), Genistein (Cat. No. 4160), Ellipticine (Cat. No. 4170)
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