Kit Replenishments

Buffers and reagents for use in TopoGEN kits.

10X Topoisomerase I Assay Buffer

10X Topo I Assay Buffer (TGS).  The buffer is optimized and tested for relaxation and cleavages by topo I.

SKU Product Size Price Buy
TG4010 10X Topoisomerase I Assay Buffer 1 ml $49.00

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5X Gyrase Assay Buffer

DNA gyrase buffer supplied as a 5X stock solution.  The buffer is optimized and tested for E. coli DNA gyrase.

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TG4030 5X Gyrase Assay Buffer 1 ml $49.00

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10X Topoisomerase IV Assay Buffer

Topo IV Assay Buffer is supplied as a 10x Stock solution in two parts that must be mixed prior to use.  The complete Topo IV Assay Buffer (A+B) is optimized and tested for decatenation and relaxation activity of E. coli topo IV.

SKU Product Size Price Buy
TG4032 10X Topoisomerase IV Assay Buffer 1 ml (A), 100 ul (B) $49.00

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5X Universal Stop Buffer

5X Stop Buffer used to terminate topoisomerase reactions.

SKU Product Size Price Buy
TG4037 5X Universal Stop Buffer 1 ml $49.00

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10X Gel Loading Buffer

Agarose Gel Loading Buffer (10X)

SKU Product Size Price Buy
TG4038 10X Gel Loading Buffer 1 ml $49.00

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10X Proteinase K

Proteinase K is a subtilisin-like endolytic protease that is isolated from the saprophytic fungus Tritirachium album.  It has a high activity that is stable across a wide range of pH and temperature conditions and is suited to short digestion t

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TG4039 10X Proteinase K 0.5ml $49.00

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Incomplete Topoisomerase II Assay Buffer

Topo II assay buffer is supplied as a 10X stock solution in two parts (A+B) that must be mixed prior to use.  The 5X Complete Topo II Assay Buffer is optimized and tested for decatenation and relaxation activity of eukaryotic topo II.

SKU Product Size Price Buy
TG4040 Incomplete Topoisomerase II Assay Buffer 1 ml (A), 1 ml (B) $49.00

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10% Sodium Dodecyl Sulfate

High purity SDS is dissolved in triple glass distilled/deionized, sterile water to 10% (w/v).

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TG4060 10% Sodium Dodecyl Sulfate 1 ml $49.00

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Sarkosyl (20%)

20% Sarkosyl

SKU Product Size Price Buy
TG4065 Sarkosyl (20%) 5 ml $99.00

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Topoisomerase II Dilution Buffer

Topo II Dilution Buffer (1X).  Optimized and tested for use as a diluent for TopoGEN's type II topoisomerase.

SKU Product Size Price Buy
TG4090 Topoisomerase II Dilution Buffer 1 ml $49.00

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Topoisomerase I Dilution Buffer

Topo I Dilution Buffer (1X).  Optimized and tested for use as a diluent for recombinant human topo I enzyme from TopoGEN.

SKU Product Size Price Buy
TG4095 Topoisomerase I Dilution Buffer 1 ml $49.00

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