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Topoisomerase Assay Kits
Detailed protocols and controlled reagents for topoisomerase kits and related products. Note that enzyme is sold separately, except where otherwise indicated.
- Gyrase Assay Kit
- Human Topoisomerase II Assay Kit
- Topoisomerase I Assay Kit
- SDS-K+ Precipitation Kit (in vivo)
- Topoisomerase I In Vivo Link Kit
- Topoisomerase II In Vivo Link Kit
- SDS-K+ Precipitation Kit (in vitro)
- DNA Intercalator/Unwinding Kit
- Gyrase/Relaxed DNA Assay Kit
Gyrase Assay Kit
The DNA Gyrase Assay Kit will allow one to quantify gyrase activity in a crude mix of other proteins (other topoisomerase enzymes for example). It is ideal for characterizing inhibitors as well.
| SKU | Product | Size | Price | Buy |
|---|---|---|---|---|
| TG1003 | Gyrase Assay Kit | 100 Assays | $299.00 |  |
Human Topoisomerase II Assay Kit
The Human Topoisomerase II Assay Kit is designed for researchers who wish to measure topo II activity in a crude extract. It is highly specific and can be quantified.
| SKU | Product | Size | Price | Buy |
|---|---|---|---|---|
| TG1001-1 | Human Topoisomerase II Assay Kit | 100 Assays | $269.00 | |
| TG1001-2 | Human Topoisomerase II Assay Kit | 250 Assays | $379.00 | |
Topoisomerase I Assay Kit
The Topoiosomerase I Assay Kit is a specific assay for topo I in a crude mixture of heterologous proteins. It is generally easy to interpret an assay that quantifies topo I activity.
| SKU | Product | Size | Price | Buy |
|---|---|---|---|---|
| TG1015-1 | Topoisomerase I Assay Kit | 100 Assays | $249.00 | |
| TG1015-2 | Topoisomerase I Assay Kit | 250 Assays | $349.00 | |
SDS-K+ Precipitation Kit (in vivo)
Designed for researchers who wish to monitor topoisomerase I or II covalent binding to a DNA target in vitro AND in vivo.
| SKU | Product | Size | Price | Buy |
|---|---|---|---|---|
| TG1011-1 | SDS-K+ Precipitation Kit (in vivo) | 100 Assays | $299.00 | |
| TG1011-2 | SDS-K+ Precipitation Kit (in vivo) | 250 Assays | $379.00 | |
Topoisomerase I In Vivo Link Kit
The Topoisomerase I in vivo Link Kit offers a very powerful method to deduce whether a given drug actually affects its predicted topo target in a given cancer cell in vivo. One can quantify the activity of topoisomerase enzyme in vivo with this kit.
| SKU | Product | Size | Price | Buy |
|---|---|---|---|---|
| TG1021 | Topoisomerase I In Vivo Link Kit | 200 Assays | $399.00 | |
Topoisomerase II In Vivo Link Kit
The Topoisomerase II in vivo Link Kit provides a very powerful method to deduce whether a given drug actually affects its predicted topo target in a given cancer cell in vivo. One can quantify the activity of topoisomerase in vivo with this kit.
| SKU | Product | Size | Price | Buy |
|---|---|---|---|---|
| TG1022 | Topoisomerase II In Vivo Link Kit | 200 Assays | $469.00 | |
SDS-K+ Precipitation Kit (in vitro)
Designed for researchers who wish to monitor topoisomerase I or II covalent binding to a DNA target in vitro AND in vivo.
| SKU | Product | Size | Price | Buy |
|---|---|---|---|---|
| TG1010-1 | SDS-K+ Precipitation Kit (in vitro) | 100 Assays | $299.00 | |
| TG1010-2 | SDS-K+ Precipitation Kit (in vitro) | 250 Assays | $399.00 | |
DNA Intercalator/Unwinding Kit
The DNA Intercalator/Unwinding Kit wil reveal how a particular drug intercalates and unwinds the DNA helix.
| SKU | Product | Size | Price | Buy |
|---|---|---|---|---|
| TG1025 | DNA Intercalator/Unwinding Kit | 50 Experiments | $349.00 | |
Gyrase/Relaxed DNA Assay Kit
TopoGEN sells E. coli gyrase along with the relaxed DNA subtrate. The enzyme is very active and can be purchased in large quantities for screening studies.
| SKU | Product | Size | Price | Buy |
|---|---|---|---|---|
| TG2000G-1KIT | Gyrase/Relaxed DNA Assay Kit | 100 Assays | $469.00 | |
| TG2000G-3KIT | Gyrase/Relaxed DNA Assay Kit | 500 Assays | $899.00 | |
| TG2000G-5KIT | Gyrase/Relaxed DNA Assay Kit | 1000 Assays | $1,399.00 | |
| TG2000G-7KIT | Gyrase/Relaxed DNA Assay Kit | 2000 Assays | $1,999.00 | |
Topoisomerase Enzymes
Custom-sized quantities of Topoisomerase Enzymes are also available and attractively priced. Please contact TopoGEN for a quote.
- Human Topoisomerase II
- Mutant Human Topo I
- Human Topoisomerase I
- E. Coli DNA Gyrase (Type II Topoisomerase)
- Topoisomerase IV
- Human Tdp1
- S. Aureus DNA Gyrase
Human Topoisomerase II
Purified DNA Topoisomerase II (p170) over expressed and purified to homogeneity to a single band on SDS-PAGE. Topo II enzyme is highly purified and free of topo I contamination.
| SKU | Product | Size | Price | Buy |
|---|---|---|---|---|
| TG2000H-1 | Human Topoisomerase II | 250 Units | $249.00 | |
| TG2000H-2 | Human Topoisomerase II | 500 Units | $349.00 | |
Mutant Human Topo I
Human Topoisomerase I (mutated active site)
| SKU | Product | Size | Price | Buy |
|---|---|---|---|---|
| TG2005H-Y723F | Mutant Human Topo I | 10 ug | $299.00 | |
Human Topoisomerase I
Human Topoisomerase I, Recombinant (Wild Type Protein).
| SKU | Product | Size | Price | Buy |
|---|---|---|---|---|
| TG2005H-RC1 | Human Topoisomerase I | 500 Units | $249.00 | |
E. Coli DNA Gyrase (Type II Topoisomerase)
Bacterial DNA Gyrase (E. coli) Enzyme (Type II Topoisomerase)
| SKU | Product | Size | Price | Buy |
|---|---|---|---|---|
| TG2000G-1 | E. Coli DNA Gyrase (Type II Topoisomerase) | 100 Units | $199.00 | |
| TG2000G-3 | E. Coli DNA Gyrase (Type II Topoisomerase) | 500 Units | $499.00 | |
| TG2000G-5 | E. Coli DNA Gyrase (Type II Topoisomerase) | 1000 Units | $869.00 | |
| TG2000G-7 | E. Coli DNA Gyrase (Type II Topoisomerase) | 2000 Units | $1,349.00 | |
Topoisomerase IV
Bacterial Topoisomerase IV (E. Coli) Enzyme
| SKU | Product | Size | Price | Buy |
|---|---|---|---|---|
| TG2000EC-1 | Topoisomerase IV | 250 Units | $299.00 | |
| TG2000EC-3 | Topoisomerase IV | 500 Units | $499.00 | |
| TG2000EC-5 | Topoisomerase IV | 1000 Units | $899.00 | |
Human Tdp1
Purified Human Tyrosyl DNA Phosphodiesterase (Tdp1)
| SKU | Product | Size | Price | Buy |
|---|---|---|---|---|
| TG2004H-1 | Human Tdp1 | 250 Units | $299.00 | |
| TG2004H-2 | Human Tdp1 | 500 Units | $399.00 | |
S. Aureus DNA Gyrase
DNA Gyrase (s. aureus) Enzyme
| SKU | Product | Size | Price | Buy |
|---|---|---|---|---|
| TG2000GSA-1 | S. Aureus DNA Gyrase | 100 Units | $139.00 | |
| TG2000GSA-3 | S. Aureus DNA Gyrase | 500 Units | $499.00 | |
| TG2000GSA-5 | S. Aureus DNA Gyrase | 1000 Units | $799.00 | |
DNA Substrates
Topoisomerase enzymes are sensitive to DNA quality (for example, heavy metal contamination) and not all DNA preps will support activity. All of our DNA substrates are screened to support topoisomerase (and gyrase) activity. When you do screening assays for DNA cleavages, you must have DNA with minimal contamination of nicked forms. Fortunately, TopoGEN controls plasmid and kDNA stocks for nicked contaminants, so you don’t have to worry about wasting expensive enzymes on inferior DNA substrate.
Kinetoplast DNA (kDNA)
Kinetoplast DNA (catenated): the ideal topoisomerase II substrate.
| SKU | Product | Size | Price | Buy |
|---|---|---|---|---|
| TG2013-1 | Kinetoplast DNA (kDNA) | 25ug | $199.00 | |
| TG2013-2 | Kinetoplast DNA (kDNA) | 50ug | $299.00 | |
| TG2013-3 | Kinetoplast DNA (kDNA) | 250ug | $699.00 | |
pRYG DNA
pRYG DNA is a plasmid containing multiple clusters of topoisomerase II cleavage sites.
| SKU | Product | Size | Price | Buy |
|---|---|---|---|---|
| TG2023-2 | pRYG DNA | 20 ug | $199.00 | |
pHOT-1 DNA
pHOT-1 DNA is a high affinity Topoisomerase I cleavage substrate.
| SKU | Product | Size | Price | Buy |
|---|---|---|---|---|
| TG2030-2 | pHOT-1 DNA | 20 ug | $199.00 | |
Supercoiled pBR322 DNA (modified)
Ideal plasmid for assaying any topoisomerase.
| SKU | Product | Size | Price | Buy |
|---|---|---|---|---|
| TG2036-1 | Supercoiled pBR322 DNA (modified) | 50 ug | $229.00 | |
| TG2036-2 | Supercoiled pBR322 DNA (modified) | 250 ug | $559.00 | |
Relaxed pBR322 DNA
Relaxed pBR322 DNA - an ideal plasmid for assaying supercoiling by DNA gyrase.
| SKU | Product | Size | Price | Buy |
|---|---|---|---|---|
| TG2037-1 | Relaxed pBR322 DNA | 50 ug | $249.00 | |
| TG2037-2 | Relaxed pBR322 DNA | 250 ug | $599.00 | |
Topoisomerase Antibodies
Topoisomerase antibodies certified for use with your topo and chromatin research.
- Monoclonal Antibody to Human Topoisomerase II Alpha
- Polyclonal Antibody to Human Topoiosomerase II Beta
- Polyclonal Antibody to Human Topoisomerase III Beta
- Polyclonal Antibody to Human Topoisomerase II Alpha
- Polyclonal Antibody to Human Topoiosomerase I (Scleroderma Patient Serum)
- Polyclonal to Human Topoisomerase I (serum)
- Monoclonal to Human Topoisomerase I
- Polyclonal to Yeast Topoisomerase II (Serum)
- Monoclonal to Human Topoisomerase III Alpha
- Polyclonal to Human Tdp1
- Topoisomerase II Alpha C-Terminal Peptide
Monoclonal Antibody to Human Topoisomerase II Alpha
Monoclonal Antibody to Human Topoisomerase II Alpha is ideal for western blotting applications or for immunohistochemistry (IHC). The high temperature unmasking technique is described in our technical literature.
| SKU | Product | Size | Price | Buy |
|---|---|---|---|---|
| TG2010-1 | Monoclonal Antibody to Human Topoisomerase II Alpha | 50 Units | $369.00 | |
Polyclonal Antibody to Human Topoiosomerase II Beta
Rabbit Polyclonal Antibody to Human Topoisomerase II Beta. Directed specifically against the C-terminus of purified topo IIb. Suitable for Westerns and IHC.
| SKU | Product | Size | Price | Buy |
|---|---|---|---|---|
| TG2010-3 | Polyclonal Antibody to Human Topoiosomerase II Beta | 250 Units | $329.00 | |
Polyclonal Antibody to Human Topoisomerase III Beta
Polyclonal Antibody to Human Topoisomerase III Beta Antibody prepared against a pool of oligopeptides derived from the carboxy terminal region of human topoisomerase IIIb. Ideal for Western blotting applications but has not been tested for other applications.
| SKU | Product | Size | Price | Buy |
|---|---|---|---|---|
| TG2010-4 | Polyclonal Antibody to Human Topoisomerase III Beta | 250 Units | $329.00 | |
Polyclonal Antibody to Human Topoisomerase II Alpha
Polyclonal Antibody to Human Topoisomerase II Alpha (p170) prepared against a 16 residue oligopeptide derived from the carboxy terminal region of human topoisomerase II. This antibody is ideal for Western blotting, immunofluorescence and immunoprecipitation.
| SKU | Product | Size | Price | Buy |
|---|---|---|---|---|
| TG2011-1 | Polyclonal Antibody to Human Topoisomerase II Alpha | 250 Units | $329.00 | |
Polyclonal Antibody to Human Topoiosomerase I (Scleroderma Patient Serum)
Hight Titer Polyclonal Antibody Directed Against Human Topoisomerase I
| SKU | Product | Size | Price | Buy |
|---|---|---|---|---|
| TG2012 | Polyclonal Antibody to Human Topoiosomerase I (Scleroderma Patient Serum) | 500 Units | $299.00 | |
Polyclonal to Human Topoisomerase I (serum)
Polyclonal Antibody to Human Topo I (Non-Affinity Purified)
| SKU | Product | Size | Price | Buy |
|---|---|---|---|---|
| TG2012-2 | Polyclonal to Human Topoisomerase I (serum) | 250 ul | $229.00 | |
Monoclonal to Human Topoisomerase I
Monoclonal Antibody Optimized for Western Blotting Applications
| SKU | Product | Size | Price | Buy |
|---|---|---|---|---|
| TG2012-4 | Monoclonal to Human Topoisomerase I | 100 ug | $299.00 | |
Polyclonal to Yeast Topoisomerase II (Serum)
Polyclonal Antibody to Yeast Topo II (Non-Affinity Purified)
| SKU | Product | Size | Price | Buy |
|---|---|---|---|---|
| TG2014 | Polyclonal to Yeast Topoisomerase II (Serum) | 250 ul | $299.00 | |
Monoclonal to Human Topoisomerase III Alpha
Monoclonal antibody raised against purified recombinant protein and specific for the C-terminal region (residues 748-976) of human topo IIIa.
| SKU | Product | Size | Price | Buy |
|---|---|---|---|---|
| TG2015 | Monoclonal to Human Topoisomerase III Alpha | 50 ul | $299.00 | |
Polyclonal to Human Tdp1
Rabbit polyclonal antibody prepared against the native Tdp1 protein.
| SKU | Product | Size | Price | Buy |
|---|---|---|---|---|
| TG2016 | Polyclonal to Human Tdp1 | 250 Units | $329.00 | |
Topoisomerase II Alpha C-Terminal Peptide
16 Residue Peptide (p170) Purified C-Terminal
| SKU | Product | Size | Price | Buy |
|---|---|---|---|---|
| TG2011-2 | Topoisomerase II Alpha C-Terminal Peptide | 50 ug | $179.00 | |
Inhibitors and Poisons
Catalytic inhibitors and poisons that induce cleavage. For use in TopoGEN kits.
- Topotecan Hydrochloride (Hycamtin)
- Irinotecan (CPT-11)
- 9-Nitro-20 (S) Camptothecin (Rubitecan)
- 10-Hydroxy Camptothecin
- 9-Amino Camptothecin
- Camptothecin
- Doxorubicin (Adriacin)
- Daunomycin
- Etoposide (VP16)
- Ellipticine
- Ciproflaxin
- Amsacrine (m-AMSA)
- Genistein
Topotecan Hydrochloride (Hycamtin)
Topotecan is a semi-synthetic derivative of camptothecin and anti-tumor drug that targets topo I. As a topo I poison it induces cleavage complexes.
| SKU | Product | Size | Price | Buy |
|---|---|---|---|---|
| TG4100 | Topotecan Hydrochloride (Hycamtin) | 0.25 ml | $199.00 | |
Irinotecan (CPT-11)
Water soluble, semi-synthetic analogue of 20(S) camptothecin (CPT) with promising activity against a range of tumor types.
| SKU | Product | Size | Price | Buy |
|---|---|---|---|---|
| TG4101 | Irinotecan (CPT-11) | 0.25 ml | $109.00 | |
9-Nitro-20 (S) Camptothecin (Rubitecan)
Rubitecan (9-nitro-CPT) is a control poison/inhibitor of topo I with promising activity against a range of tumor types.
| SKU | Product | Size | Price | Buy |
|---|---|---|---|---|
| TG4102 | 9-Nitro-20 (S) Camptothecin (Rubitecan) | 0.25ml | $109.00 | |
10-Hydroxy Camptothecin
A control poison/inhibitor of topo I with promising activity against a range of tumor types.
| SKU | Product | Size | Price | Buy |
|---|---|---|---|---|
| TG4103 | 10-Hydroxy Camptothecin | 0.25 ml | $109.00 | |
9-Amino Camptothecin
This is an analog of camptothecin showing promising activity against a range of tumor types. It is provided as a control poison/inhibitor of topo I.
| SKU | Product | Size | Price | Buy |
|---|---|---|---|---|
| TG4104 | 9-Amino Camptothecin | 0.25 ml | $109.00 | |
Camptothecin
This topo I poison both inhibits enzyme activity and stabilizes formation of topo I cleavage complexes.
| SKU | Product | Size | Price | Buy |
|---|---|---|---|---|
| TG4110 | Camptothecin | Lyophilized | $99.00 | |
Doxorubicin (Adriacin)
Doxorubicin (Adriacin) is a control poison/inhibitor of topo II.
| SKU | Product | Size | Price | Buy |
|---|---|---|---|---|
| TG4120 | Doxorubicin (Adriacin) | 0.25 ml | $109.00 | |
Daunomycin
This is a control poison/inhibitor of topo II.
| SKU | Product | Size | Price | Buy |
|---|---|---|---|---|
| TG4130 | Daunomycin | 0.25 ml | $99.00 | |
Etoposide (VP16)
This is a control poison/inhibitor of topo II. It is also included in the topo II drug screening kit.
| SKU | Product | Size | Price | Buy |
|---|---|---|---|---|
| TG4140 | Etoposide (VP16) | Lyophilized | $99.00 | |
Ellipticine
Ellipticine is a topo II poison that induces topo II dependent cleavages.
| SKU | Product | Size | Price | Buy |
|---|---|---|---|---|
| TG4170 | Ellipticine | 0.25 ml | $99.00 | |
Ciproflaxin
A control poison/inhibitor of DNA Gyrase and Topo IV. This is also included in the Topo IV/Gyrase Drug Screening Kits.
| SKU | Product | Size | Price | Buy |
|---|---|---|---|---|
| TG4180 | Ciproflaxin | 50 ul | $69.00 | |
Amsacrine (m-AMSA)
This is a control poison/inhibitor of topoisomerase II.
| SKU | Product | Size | Price | Buy |
|---|---|---|---|---|
| TG4150 | Amsacrine (m-AMSA) | 0.25 ml | $99.00 | |
Genistein
Isoflavone and inhibitor of tyrosine protein kinases reported to induce topo II dependent cleavages.
| SKU | Product | Size | Price | Buy |
|---|---|---|---|---|
| TG4160 | Genistein | 0.25 ml | $99.00 | |
Protein MW Markers
Molecular weight markers for proteins - available from TopoGEN.
Human Topoisomerase II Alpha
Purified p170 kDa Sufficient for 20 Gel Loads
| SKU | Product | Size | Price | Buy |
|---|---|---|---|---|
| TG2011-3 | Human Topoisomerase II Alpha | 20 Units | $199.00 | |
Topoisomerase I Western Blot
Western Blot Marker Set for Topo I
| SKU | Product | Size | Price | Buy |
|---|---|---|---|---|
| TG2011-4 | Topoisomerase I Western Blot | 20 Units | $169.00 | |
Protocols/Reference
A two volume set of experimental protocols is available from Humana. These protocols are a must for any lab involved in topoisomerase related research. If your lab is already established or if you are just getting set up, these books provide new, incoming students and post-docs a thorough and complete resource for any topo-based procedure. A must for every lab in the field.
DNA Topoisomerase Protocols Volume I
DNA Topoisomerase Protocols Volume I
DNA Topology and Enzymes
Eds. M.Bjornsti and N.Osheroff
Humana Press
| SKU | Product | Size | Price | Buy |
|---|---|---|---|---|
| TGVI | DNA Topoisomerase Protocols Volume I | N/A | $129.00 | |
DNA Topoisomerase Protocols Volume II
DNA Topoisomerase Protocols Volume II
Enzymology and Drugs
Eds. M.Bjornsti and N.Osheroff
Humana Press
| SKU | Product | Size | Price | Buy |
|---|---|---|---|---|
| TGVII | DNA Topoisomerase Protocols Volume II | N/A | $129.00 | |
Kit Replenishments
Buffers and reagents for use in TopoGEN kits.
- 10X Topoisomerase I Assay Buffer
- 5X Gyrase Assay Buffer
- 10X Topoisomerase IV Assay Buffer
- 5X Universal Stop Buffer
- 10X Gel Loading Buffer
- 10X Proteinase K
- Incomplete Topoisomerase II Assay Buffer
- 10% Sodium Dodecyl Sulfate
- Sarkosyl (20%)
- Topoisomerase II Dilution Buffer
- Topoisomerase I Dilution Buffer
10X Topoisomerase I Assay Buffer
10X Topo I Assay Buffer (TGS). The buffer is optimized and tested for relaxation and cleavages by topo I.
| SKU | Product | Size | Price | Buy |
|---|---|---|---|---|
| TG4010 | 10X Topoisomerase I Assay Buffer | 1 ml | $49.00 | |
5X Gyrase Assay Buffer
DNA gyrase buffer supplied as a 5X stock solution. The buffer is optimized and tested for E. coli DNA gyrase.
| SKU | Product | Size | Price | Buy |
|---|---|---|---|---|
| TG4030 | 5X Gyrase Assay Buffer | 1 ml | $49.00 | |
10X Topoisomerase IV Assay Buffer
Topo IV Assay Buffer is supplied as a 10x Stock solution in two parts that must be mixed prior to use. The complete Topo IV Assay Buffer (A+B) is optimized and tested for decatenation and relaxation activity of E. coli topo IV.
| SKU | Product | Size | Price | Buy |
|---|---|---|---|---|
| TG4032 | 10X Topoisomerase IV Assay Buffer | 1 ml (A), 100 ul (B) | $49.00 | |
5X Universal Stop Buffer
5X Stop Buffer used to terminate topoisomerase reactions.
| SKU | Product | Size | Price | Buy |
|---|---|---|---|---|
| TG4037 | 5X Universal Stop Buffer | 1 ml | $49.00 | |
10X Gel Loading Buffer
Agarose Gel Loading Buffer (10X)
| SKU | Product | Size | Price | Buy |
|---|---|---|---|---|
| TG4038 | 10X Gel Loading Buffer | 1 ml | $49.00 | |
10X Proteinase K
Proteinase K is a subtilisin-like endolytic protease that is isolated from the saprophytic fungus Tritirachium album. It has a high activity that is stable across a wide range of pH and temperature conditions and is suited to short digestion t
| SKU | Product | Size | Price | Buy |
|---|---|---|---|---|
| TG4039 | 10X Proteinase K | 0.5ml | $49.00 | |
Incomplete Topoisomerase II Assay Buffer
Topo II assay buffer is supplied as a 10X stock solution in two parts (A+B) that must be mixed prior to use. The 5X Complete Topo II Assay Buffer is optimized and tested for decatenation and relaxation activity of eukaryotic topo II.
| SKU | Product | Size | Price | Buy |
|---|---|---|---|---|
| TG4040 | Incomplete Topoisomerase II Assay Buffer | 1 ml (A), 1 ml (B) | $49.00 | |
10% Sodium Dodecyl Sulfate
High purity SDS is dissolved in triple glass distilled/deionized, sterile water to 10% (w/v).
| SKU | Product | Size | Price | Buy |
|---|---|---|---|---|
| TG4060 | 10% Sodium Dodecyl Sulfate | 1 ml | $49.00 | |
Sarkosyl (20%)
20% Sarkosyl
| SKU | Product | Size | Price | Buy |
|---|---|---|---|---|
| TG4065 | Sarkosyl (20%) | 5 ml | $99.00 | |
Topoisomerase II Dilution Buffer
Topo II Dilution Buffer (1X). Optimized and tested for use as a diluent for TopoGEN's type II topoisomerase.
| SKU | Product | Size | Price | Buy |
|---|---|---|---|---|
| TG4090 | Topoisomerase II Dilution Buffer | 1 ml | $49.00 | |
Topoisomerase I Dilution Buffer
Topo I Dilution Buffer (1X). Optimized and tested for use as a diluent for recombinant human topo I enzyme from TopoGEN.
| SKU | Product | Size | Price | Buy |
|---|---|---|---|---|
| TG4095 | Topoisomerase I Dilution Buffer | 1 ml | $49.00 | |
DNA Molecular Weight Markers
DNA molecular weight markers for agarose gels. For use in TopoGEN kits.
Linear kDNA
Linear Kinetoplast DNA Marker
| SKU | Product | Size | Price | Buy |
|---|---|---|---|---|
| TG2017-1 | Linear kDNA | 10 ug | $169.00 | |
Decatenated kDNA
Decatenated Kinetoplast DNA Marker
| SKU | Product | Size | Price | Buy |
|---|---|---|---|---|
| TG2020-1 | Decatenated kDNA | 10 ug | $189.00 | |
Linear pRYG DNA
Linear pRYG DNA Marker
| SKU | Product | Size | Price | Buy |
|---|---|---|---|---|
| TG2028-1 | Linear pRYG DNA | 10 ug | $109.00 | |
Relaxed pRYG DNA
Relaxed pRYG DNA Marker
| SKU | Product | Size | Price | Buy |
|---|---|---|---|---|
| TG2025-1 | Relaxed pRYG DNA | 10 ug | $149.00 | |
Relaxed pHOT-1 DNA
Relaxed pHOT-1 DNA Marker
| SKU | Product | Size | Price | Buy |
|---|---|---|---|---|
| TG2035-1 | Relaxed pHOT-1 DNA | 10 ug | $129.00 | |
Drug Screening Kits
Drug Screening Kits to demonstrate how a novel compound affects topoisomerase action from a mechanistic standpoint. Detailed protocols and all necessary controlled reagents are included. Note that enzyme is sold separately.
- Topoisomerase IV Drug Screening Kit
- Topoisomerase II Drug Screening Kit
- Topoisomerase I Drug Screening Kit
Topoisomerase IV Drug Screening Kit
The Topoisomerase IV Drug Screeing Kit is designed for researchers who wish to measure the realease of topoisomerase from covalent complexes that form as a result of drug treatment. It is highly specific and can be quantified.
| SKU | Product | Size | Price | Buy |
|---|---|---|---|---|
| TG1007 | Topoisomerase IV Drug Screening Kit | 100 Assays | $239.00 | |
Topoisomerase II Drug Screening Kit
The Topoisomerase II Drug Screening Kit will help you interpret how a novel drug affects topo II action.
| SKU | Product | Size | Price | Buy |
|---|---|---|---|---|
| TG1009-1 | Topoisomerase II Drug Screening Kit | 100 Assays | $269.00 | |
| TG1009-2 | Topoisomerase II Drug Screening Kit | 250 Assays | $379.00 | |
Topoisomerase I Drug Screening Kit
The Topoisomerase I Drug Screening Kit allows the researcher to model drug action with novel topo I active agents. For example, is it a poison or an inhibitor?
| SKU | Product | Size | Price | Buy |
|---|---|---|---|---|
| TG1018-1 | Topoisomerase I Drug Screening Kit | 100 Assays | $269.00 | |
| TG1018-2 | Topoisomerase I Drug Screening Kit | 250 Assays | $369.00 | |
Antibody Kits
Topoisomerase Antibody-based kits available from TopoGEN.
Topoisomerase II Immunoflourescence Kit
Cytologically track topo II in cells with the Topoisomerase II Immunoflourescence Kit. Controls are included along with detailed documentation on use of TopoGEN antibodies.
| SKU | Product | Size | Price | Buy |
|---|---|---|---|---|
| TG1030 | Topoisomerase II Immunoflourescence Kit | 50 Analyses | $399.00 | |
Topoisomerase II Immunoprecipitation Kit
Those interested in protein/protein interactions will find the Topoisomerase II Immunoprecipitation Kit useful.
| SKU | Product | Size | Price | Buy |
|---|---|---|---|---|
| TG1035 | Topoisomerase II Immunoprecipitation Kit | 50 Analyses | $399.00 | |
Tdp1 Products
Tdp1 and related products available from TopoGEN.
Tdp1 Assay Kit
The Tdp1 Assay kit is designed for researchers who wish to measure the release of topoisomerase from covalent complexes that form as a result of drug treatment. It is highly specific and can be quantified.
| SKU | Product | Size | Price | Buy |
|---|---|---|---|---|
| TG1004-1 | Tdp1 Assay Kit | 100 Assays | $249.00 | |
| TG1004-2 | Tdp1 Assay Kit | 250 Assays | $349.00 | |