614-451-5810 
Monoclonal to Human Topoisomerase I
| SKU | Product | Size | Price | Buy |
|---|---|---|---|---|
| TG2012-4 | Monoclonal to Human Topoisomerase I | 100 ug | $299.00 |  |
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Methods-for-Extracting-Topoisomerases-for-Western-Blots |
This monoclonal antibody was raised to human topo I (TG2005H-RC1) using standard methods. The antibody (IgG class) works very well for Western blotting experiments. The epitope has not been mapped. This product has not been tested for IP or IF applications; however, given the high specificity seen on Westerns, it should work well in these applications.
Viral Contents:
100 ug of purified monoclonal antibody (concentration at 10 ug/ul; total volume of 10 ul). For Western blotting applications, we recommend 1:10,000 dilution when probing crude nuclear extracts. A single prominent band of 100 kDa was detected using fresh nuclear extracts. Since topo I can degrade, we recommend using fresh extracts.
Shipping and Storage Instructions:
The antibody is stable at room temperature but should be stored at -20°C. The antibody is shipped at ambient temperature.