614-451-5810 
Topoisomerase I Assay Kit
| SKU | Product | Size | Price | Buy |
|---|---|---|---|---|
| TG1015-1 | Topoisomerase I Assay Kit | 100 Assays | $249.00 |  |
| TG1015-2 | Topoisomerase I Assay Kit | 250 Assays | $349.00 | |
| Related Products | Related Documents |
|---|---|
Topoisomerase I Assay Kit Product Description
This kit contains reagents for routine detection of type I topoisomerase activity. DNA markers (included) allow unequivocal detection of the enzyme. The assay is specific for topoisomerase I; (eukaryotic type II topoisomerases require MgCl2 and ATP). The standard topo I assay kit is supplied with EDTA containing reaction buffer (minus Mg++) in order to prevent nucleolytic nicking of the supercoiled substrate (although we note that some eukaryotic type I activities are stimulated by Mg++). This assay will work even with crude extracts. A detailed manual of instruction (see link above) is included that describes how to run the gels to maximally resolve topoisomers for analysis of DNA unwinding.
Topoisomerase I Assay Kit Contents (representative for 100 assay kit size):
- Supercoiled pHOT-1 DNA (25 µg in 100 µl TE buffer, or 0.25 µg/ul)
- Relaxed pHOT-1 DNA marker, relaxed by topo I (50 ul at 0.05 µg/ml in gel loading buffer)
- 10X Topo I assay buffer (300 µl)
- 5X Stop buffer/gel loading dye (600 µl)
- Detailed instruction manual
- Sample data and documentation of all controls
Reviews and Citations:
- Dexheimer TS, Pommier Y: DNA cleavage assay for the identification of topoisomerase I inhibitors. Nature 2008, 3(11): 1736-1750. doi:10.1038/nprot.2008.174
- Zhang L, Sullivan P, Suyama J, et. al: Epidermal growth factor-induced heparanase nucleolar localization augments DNA topoisomerase I activity in brain metastatic breast cancer. Molecular Cancer Research 2010, 8: 278-290. doi: 10.1158/1541-7786.MCR-09-0375
Shipping and Storage Instructions:
This kit is shipped at ambient temperature and should be stored at 4°C.