The immunoprecpitation (IP) kit contains the key reagents and protocol for immunoprecipitating p170 human topoisomerase II. The method should work well with other cells besides humans. For example, mouse, rat and monkey cells will cross react withtopoisomerase II antibody. The antibody is specific for p170 (topo II alpha) in human (HeLa) cells and although we presume it will behave similarly in other species with cross reactive topo II isozymes, this should be carefully evaluated.
Anti-topo II (p170) Antibody, (TG2011-1). This is a rabbit antibody (polyclonal) directed against the C-Terminal region of p170. A peptide was used to raise this antiserum; the antibody has been immunoaffinity purified. The antibody is specific for topo II alpha (p170) form. Kit contains 100 ul of antibody.
Human topo II p170 Marker . The marker is ready to load in 1X SDS PAGE Loading buffer. Loading 8-10 ul of this marker will give a readily detectable band on commassie blue staining. Kit contains 100 ul of marker.
Detailed Protocol for immunoprecipitation
Detailed Protocol for preparation of nuclear extracts
Topo I Enzyme Provided for Autoimmunity Study
Immunization with DNA topoisomerase I and Freund's complete adjuvant induces skin and lung fibrosis and autoimmunity via interleukin-6 signaling.
The presence of anti-DNA topoisomerase I (anti-topo I) anti...