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Month: November 2010

Nuclease Assays with pN01 from TopoGEN

Nuclease Assays with pN01 from TopoGEN

Saccharomyces cerevisiae Mre11/Rad50/Xrs2 and Ku proteins regulate association of Exo1 and Dna2 with DNA breaks Eun Yong Shim1,4, Woo-Hyun Chung2,4, Matthew L Nicolette3, Yu Zhang1, Melody Davis1, Zhu Zhu2, Tanya T Paull3, Grzegorz Ira2,* and Sang Eun Lee1,* 1Department of Molecular Medicine, Institute of Biotechnology, University of Texas Health Science Center at San Antonio, San Antonio, TX, USA, 2Department of Molecular and Human Genetics, Baylor College of Medicine, One Baylor Plaza, Houston, TX, USA and 3Department of Molecular Genetics and…

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Upcoming Holiday Closure

Upcoming Holiday Closure

TopoGEN will be closed for shipping November 24-26 for Thanksgiving. Our last official ship-day before Christmas is December 20. We will resume normal shipping operation on January 10, 2011. During this time our staff will remain available via e-mail to respond to technical questions, internet orders, and general information. Feel free to drop us a line.

Topoiosomerase Inhibition: A Study

Topoiosomerase Inhibition: A Study

Cytotoxicity and topoisomerase I/II inhibition of glycosylated 2-phenyl-indoles, 2-phenyl-benzo[b]thiophenes and 2-phenyl-benzo[b]furans Wei Shia, Sandra L. Marcusa and Todd L. Lowary Alberta Ingenuity Centre for Carbohydrate Science and Department of Chemistry, The University of Alberta, Gunning-Lemieux Chemistry Centre, Edmonton, AB T6G 2G2 Canada Abstract A panel of glycosylated DNA binding agents (1–12) designed as functional anthracycline mimics was screened against three solid-tumor cell lines (MCF-7, HT 29 and HepG2/C3A) and three non-tumor cell lines by the MTS (3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium) cell viability assay….

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DNA Unwinding Kit Controls DNA Binding Modes

DNA Unwinding Kit Controls DNA Binding Modes

Substituent control of DNA binding modes in a series of chalcogenoxanthylium photosensitizers as determined by isothermal titration calorimetry and topoisomerase I DNA unwinding assay References and further reading may be available for this article. Ruel E. McKnighta, Corresponding Author Contact Information, E-mail The Corresponding Author, Bilgehan Onogula, Shivani R. Polasania, Michael K. Gannon IIb and Michael R. Dettyb, Corresponding Author Contact Information, E-mail The Corresponding Author Department of Chemistry, State University of New York at Geneseo, 1 College Circle, Geneseo,…

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