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Month: February 2012

Study: Geminin Silencing Inhibits Topo II Function

Study: Geminin Silencing Inhibits Topo II Function

The Topo II Assay Kit is arguably our most simple and tractable diagnostic, making it one of our most popular products for novel chemo-therapuetics research.  Referenced here is a study that utilized the assay to monitor topoisomerase II enzyme in relation to the presence of geminin in human breast cancer cells.  The findings indicate that targeting geminin has therapeutic potential in the study of cancer cells and drug resistance.  This work opens up the prospect of using geminin as a…

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Emodin: Topo II Inhibitor and Poison

Emodin: Topo II Inhibitor and Poison

In this work, the authors demonstrate very nicely the mode of action of emodin, an active anthraquinone natural product from plants and fungi (and used as a laxative).  This widely used drug is genotoxic and is a carcinogen.  Its mode of action is unclear and this work defines it as a topo II inhibitor and poison.  They propose a mechanism involving both cleavage stabilization and inhibition of ATP hydrolysis.  The key experiments involved using the ICE bioassay to examine in…

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