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Month: December 2012

Quantifying Topo Action in E. Coli

Quantifying Topo Action in E. Coli

A new method to quantify endogenous Topoisomerase action in E. coli cells is nicely described in this paper. The technique is based on the In Vivo Link Kit, also known as the ICE bioassay. Article Source: American Society for Microbiology Isolation and Quantitation of Topoisomerase Complexes Accumulated on Escherichia coli Chromosomal DNA Abstract DNA topoisomerases are important targets in anticancer and antibacterial therapy because drugs can initiate cell death by stabilizing the transient covalent topoisomerase-DNA complex. In this study, we…

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