Revealing new paper on metallo-inhibitors. Article Source: Science Direct Synthesis and structure elucidation of a cobalt(II) complex as topoisomerase I inhibitor: In vitro DNA binding, nuclease and RBC hemolysis Abstract Metal-based cancer chemotherapeutic agent of the type Co(II) complex [Co(mpca)2]·H2O (1), where, Hmpca = 9-methyl-[1,10]phenanthroline-2-carboxylic acid was synthesized and characterized by various spectroscopic and analytical techniques and further authenticated by single crystal X-ray diffraction methods. In vitro DNA binding studies of complex 1 with CT DNA was carried out by…

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A new reverse gyrase from Pyrobaculum species has been identified with some novel properties. It combines helicase with topoisomerase domains. Very interesting from an evolutionary perspective. Article Source: The Journal of Biological Chemistry The reverse gyrase form Pyrobaculum calidifontis, a novel extremely thermophilic DNA topoisomerase endowed with DNA unwinding and annealing activities Abstract Reverse gyrase is a DNA topoisomerase specific of hyperthermophilic bacteria and archaea. It catalyses the peculiar ATP-dependent DNA positive supercoiling reaction and might be involved in the…

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Topology is critical in allowing chromatin to function in a normal manner. DNA templating is clearly impacted when enzymes that relieve strain are blocked in a chromatin context. Specifically, inhibition of topoisomerases leads to rapid accumulation of torsional strain accompanied by changes in Pol II kinetics and destabilization of nucleosomes. Stress іѕ аn inevitable раrt оf life аnd саn bесоmе especially acute аѕ wе age duе tо thе stresses associated wіth family, work, аnd financial responsibilities. Thе older wе gеt…

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The authors have used a clever genetic screen to identify “Cheater mutations” in genes that confer the ability of cells to behave in a non-cooperative fashion in a tissue context. These cheater cells could for example, outgrow surrounding cells as aberrant clones, and thereby derail a tightly regulated process of differentiation that is pivotal in development. A very nice bioinformatic rendering of the data revealed that an axis of regulatory genes that are under the influence of topoisomerase I and…

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