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Month: December 2014

Topo I Assay Quickstart: How to get the assay properly validated and established in my lab?

Topo I Assay Quickstart: How to get the assay properly validated and established in my lab?

Whether you are conducting your first topo I specific assay or you have multiple publications identifying novel topoisomerase inhibitors, you are aware that working with catalytically active proteins can involve extra time constraints if you do not first validate the working conditions of your assay. The following simple experiment should be performed when you receive your human topoisomerase I enzyme and topoisomerase I assay kit. You must show that in your lab, you can detect topo activity. This experiment is…

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