Topoisomerases and Enzymatic Activity: A How-To Guide to Establishing Activity
If you experience low or no activity with our enzymes, consider doing a ‘reconstruction’ type of experiment as follows. Note that this is generally good practice upon receipt of new enzyme from TopoGEN, just to verify that everything is working properly in your lab. FIRST, When you receive your product, be sure to store as directed by the product literature. Often this means placing immediately at -70ºC. In some cases you can thaw and aliquot the enzyme (not less than…