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Cell Context DNA Repair Kits Now Available

Cell Context DNA Repair Kits Now Available

Are you working on DNA-damage and repair?  Would you be interested in a pre-validated cell-context system to analyze HR or NHEJ by single cell imaging or quantifying DNA repair by simple fluorescence?
TopoGEN, Inc. has developed a suite of cell lines that will allow you to study specific DNA repair pathways in living cells.
Our systems report specific Homologous Recombination (HR) well as Non-Homologous End Joining (NHEJ) pathways.  These engineered cell lines are truly novel, giving unprecedented insight into HR and NHEJ at a single cell level of resolution.  DNA repair is activated using an inducible DNA damage event directed specifically at a neutral reporter gene.  Moreover, since only repaired cells become fluorescent, the investigator can follow each individual cell (and all descendants) by live imaging.
We offer certified cell lines hosting both HR and NHEJ in human and mouse species.  There are neuron specific and fibroblast hosting lines with other cell lineages under development (cardiac, kidney, NCI-60 lines, IPSCs).
You can also provide us with your unique cell line to ‘host’ these repair systems (see our “Custom Services” program where we install an HR or NHEJ reporter in your specific cell line). Please contact us for a quote.

To learn more about specific cell line reporters, see the following:
Homologous Recombination Repair Cell Line Reporters (Catalog Number)

DR3000 HRHeLa-Sce              HR based Kit in HeLa (Includes I-Sce1 plasmid for transfection)

DR3000 HRHeLa-CPR             HR based Kit in HeLa (Includes CRISPR-Cas plasmid for inducing DNA breaks)

DR3000 HRCN1.4-Sce            HR based Kit in Mouse Neuronal Cells (Includes I-Sce1 plasmid for transfection)

DR3000 HRSH-Sce                  HR in Human Neuroblastoma Cells (Includes I-Sce1 plasmid for transfection)

DR3000 HR3T3-Sce                HR based Kit in Mouse fibroblast 3T3 Cells (Includes I-Sce1 plasmid for transfection)

*DR3000 Custom-Sce            Custom service: make your own HR system your specific cell line

DR3001 iHRHeLa-Tet             HR based in HeLa uses Tet-on (Dox inducible) to initiate DNA damage with I-Sce1

Non-Homologous End Joining DNA Repair Cell Line Reporters (Catalog Number)

DR5000 HNHeLa-Sce             NHEJ based Kit in HeLa (Includes I-Sce1 plasmid for transfection)

DR5000 HNSH-Sce                  NHEJ in Human Neuroblastoma Cells (Includes I-Sce1 plasmid for transfection)

DR5000 HNCN1.4-Sce           NHEJ based Kit in Mouse Neuronal Cells (Includes I-Sce1 plasmid for transfection)

DR5000 HN3T3-Sce                NHEJ based Kit in Mouse fibroblast 3T3 Cells (Includes I-Sce1 plasmid for transfection)

*DR5000-Custom                    Custom service: make your own NHEJ system your specific cell line

DR5001- iHNHela-Tet            NHEJ based in HeLa uses Tet-on (Dox inducible) to initiate DNA damage with I-Sce1
HR & NHEJ in same cell:
Multiplex Systems:  Analyze HR (GFP) and NHEJ (YFP) in the same cell line (please enquire).

*Custom Kits allow you, the customer to create a project or disease specific cell line to host HR or NHEJ.
Alternatively, we can make the cell line for you (please enquire).

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