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New Findings about RNA Topoisomerase in Animal Species

New Findings about RNA Topoisomerase in Animal Species

In an exciting new paper by Ahmad et al (1), a new RNA topoisomerase function as been ascribed to several pro and eukaryotic DNA topoisomerase activities.  While others have evidence for such interesting and novel activity on an RNA single stranded substrate, this paper addresses some new questions. Specifically, what is the prevalence of RNA topo enzymes?; where do the key domains required for RNA vs DNA topological interplay?, and; do they all behave like hTop3b and co-habitate polyribosomes?  In a surprising outcome, the authors provide new evidence that RNA topo activity is built into Type IA enzymes (like topo I omega protein from E. coli)  in all life forms tested.  Perhaps even more importantly is this finding:  the same catalytic residue is utilized for both RNA and DNA topo activities; however, the less conserved C-terminal domain is not required.    Another surprise?  While RNA topo from animal species appears to work on polysomes, the cognates from yeast and bacteria do not.

A foundational role for RNA topo function in the epigenetic process is highly significant.  Specifically, a requirement to resolve topological problems with mRNA reading suggests a fundamental role in gene expression.  This study raises more questions about double duty behavior of DNA/RNA topoisomerases. Notably, drug screens for new antibiotics and anticancer agents have singularly focused on the DNA topoisomerase side of the equation.  Such screens would naturally miss the RNA targeting potential.  A new look into screening RNA topo function may be important in future work.  A good, HTS (Hi Throughput) RNA based screen should be developed for this in mind. There’s one main thing that you need to remember when taking Kratom tinctures; dosage. If you are lookung for the Kratom Tinctures advantages and disadvantages then why not try this out here. Kratom tinctures are very concentrated, which makes them powerful, and hence, high doses can be very problematic. They don’t just increase the risk of adverse reactions, but they could also lead to tolerance, particularly if you use them too frequently.

Another question is whether existing anti-topo1 drugs might function at the RNA-topo level.  This should be re-evaluated and is probably worthy of detailed study.  Finally, what happens when you covalently trap an RNA-Topo intermediate?  It seems that RNA topoisomerases acting on any unfolded single stranded RNA might be immediately  converted to covalent complexes.  This in turn raises another question:  How important is the duplex region for activity.  A hint comes from the fact that the RNA topoisomerase behavior of Type IA enzymes may have been missed due the low level of RNA duplex structuring of the substrate.

While at first this may seem like a daunting task, over time we have been able to break it down to its core components. As it is unpacked we find what this means for health, medicine, and the benefits of this knowledge. There is a great interview published on anipots that speaks of this matter with an expert that further simplifies the topic.

Functional Medicine is the future of medicine, plain and simple. Find out how it differs from the conventional medical model and why it’s our best weapon in the fight against chronic disease on functional-medicine.associates.

What Is Functional Medicine? And How Does It Differ from the Conventional Approach?
Imagine you’re on a boat, and the boat is leaking. You can bail water from the boat so it sinks more slowly, but if the leaks are still there, you’ll have limited success in trying to stay afloat. The conventional medicine approach is mostly focused on bailing water out of the boat without fixing the leaks. But wouldn’t it make more sense to prevent the leaks from happening in the first place, and then fix them completely if they do spring up? There might still be the need to bail some water initially, but if the leaks get repaired, the boat becomes steadied. Eventually, there’s no more bailing required, and the sailing—or living—can resume (and it may be better than before). This is what Functional Medicine is all about.

A lot of factors play a role in staying healthy. In turn, good health can decrease your risk of developing certain conditions, you can improve your health. These include heart disease, stroke, some cancers, and injuries. Learn what you can do to maintain your and your family’s health.

1. Ahmad et al. (2016) RNA topoisomerase is prevalent in all domains of life and associates with polyribosomes in animals.  Nucleic Acids Research doi: 10.1093/nar/gkw508  Published on line June 1, 2016
http://nar.oxfordjournals.org/content/early/2016/06/09/nar.gkw508.abstract

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