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Cell Context DNA Repair Kits Now Available

Cell Context DNA Repair Kits Now Available

Are you working on DNA-damage and repair?  Would you be interested in a pre-validated cell-context system to analyze HR or NHEJ by single cell imaging or quantifying DNA repair by simple fluorescence? TopoGEN, Inc. has developed a suite of cell lines that will allow you to study specific DNA repair pathways in living cells. Our systems report specific Homologous Recombination (HR) well as Non-Homologous End Joining (NHEJ) pathways.  These engineered cell lines are truly novel, giving unprecedented insight into HR…

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Mitigating Chemotherapy Resistance to Irinotecan, A Popular Topo I Inhibitor used in Treatment of Breast and Colon Cancer

Mitigating Chemotherapy Resistance to Irinotecan, A Popular Topo I Inhibitor used in Treatment of Breast and Colon Cancer

Researchers at the University of Sheffield have been investigating the causes for chemotherapy resistance in colon and breast cancer.  Their findings, recently published in Nucleic Acids Research, reveal that there is an epigenetically-driven process that speeds up the repair of DNA breakage caused during the course of chemotherapy, in this case with use of the Topo I inhibitor Irinotecan.  This process is referred to as “histone acetylation” and is treatable with clinically available therapeutics (HDAC Inhibitors). Meisenberg, et al. (2016)…

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New Findings about RNA Topoisomerase in Animal Species

New Findings about RNA Topoisomerase in Animal Species

In an exciting new paper by Ahmad et al (1), a new RNA topoisomerase function as been ascribed to several pro and eukaryotic DNA topoisomerase activities.  While others have evidence for such interesting and novel activity on an RNA single stranded substrate, this paper addresses some new questions. Specifically, what is the prevalence of RNA topo enzymes?; where do the key domains required for RNA vs DNA topological interplay?, and; do they all behave like hTop3b and co-habitate polyribosomes?  In…

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Exciting New Findings on Top1 as Target for Mitigating the Proinflammatory Response

Exciting New Findings on Top1 as Target for Mitigating the Proinflammatory Response

See Rialdi et al., Science 352 aad7993 Summary Topoisomerase I (Top1) represents an exciting new target in regulating host defense against pathogenic insult. Specifically, the balance between pro- and anti-inflammatory outcomes depends critically on equilibrium levels of endogenous Top1. Pharmacological intervention of Top1 action in chromatin therefore represents a novel and exciting inroad in treating acute inflammation which can be lethal in some cases. Why is this exciting? The innate immune response is a pivotal and early onset protection mechanism…

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TopoGEN Awarded Advanced Industry Accelerator Grant by Colorado OEDIT

TopoGEN Awarded Advanced Industry Accelerator Grant by Colorado OEDIT

It pleases us immensely to announce that TopoGEN has been named by the Colorado Office of Economic Development and International Trade (OEDIT) as a grantee of the Advanced Industry Accelerator Grant Program. In 2015 we began the task of moving our entire operation into the Colorado Rockies.  While the relocation of a wet lab and business office halfway across the country has not been without some requisite hardships, our staff has never lost sight of our goal: bring Cancer Research…

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Topoisomerases and Enzymatic Activity: A How-To Guide to Establishing Activity

Topoisomerases and Enzymatic Activity: A How-To Guide to Establishing Activity

If you experience low or no activity with our enzymes, consider doing a ‘reconstruction’ type of experiment as follows. Note that this is generally good practice upon receipt of new enzyme from TopoGEN, just to verify that everything is working properly in your lab. FIRST, When you receive your product, be sure to store as directed by the product literature. Often this means placing immediately at -70ºC. In some cases you can thaw and aliquot the enzyme (not less than…

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Topo I Assay Quickstart: How to get the assay properly validated and established in my lab?

Topo I Assay Quickstart: How to get the assay properly validated and established in my lab?

Whether you are conducting your first topo I specific assay or you have multiple publications identifying novel topoisomerase inhibitors, you are aware that working with catalytically active proteins can involve extra time constraints if you do not first validate the working conditions of your assay. The following simple experiment should be performed when you receive your human topoisomerase I enzyme and topoisomerase I assay kit. You must show that in your lab, you can detect topo activity. This experiment is…

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