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Decatenation Assays and Topo II – Supercoiled pBR322 Provided by TopoGEN

Decatenation Assays and Topo II – Supercoiled pBR322 Provided by TopoGEN

A non-canonical function of topoisomerase II  in disentangling dysfunctional telomeres Thomas Germe, Kyle Miller1 and  Julia Promisel Cooper Telomere Biology Laboratory, Cancer Research, London, UK The decatenation activity of topoisomerase II (Top2), which is widely conserved within the eukaryotic domain, is essential for chromosomal segregation in mitosis.  It is less clear, however, whether Top2 performs the same function uniformly across the whole genome, and whether all its functions rely on decatenation.  In the fission yeast, Schizosaccharomyces pombe, telomeres are bound…

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New Solid Phase Assay Coming Soon

New Solid Phase Assay Coming Soon

TopoGEN scientists have developed a novel solid phase screening assay suitable for High Throughput Screening. This method has the ability to unambiguously identify topoisomerase targeting drugs. In addition, this HTS method will predict if a test drug is an INTERFACIAL POISON or a CATALYTIC INHIBITOR. Stay tuned for more details! The paper has been submitted for publication and will be released on this blog. In the meantime feel free to contact us with any questions about this exciting new development.

Findings of PARP Inhibitors Study at NIH

Findings of PARP Inhibitors Study at NIH

Poly(ADP-ribose) polymerase and XPF-ERCC1 participate in distinct pathways for the repair of topoisomerase I-induced DNA damage in mammalian cells. Zhang YW, Regairaz M, Seiler JA, Agama KK, Doroshow JH, Pommier Y. Laboratory of Molecular Pharmacology, Center for Cancer Research, National Cancer Institute, National Institute of Health, Bethesda, MD 20892, USA. Abstract Poly(ADP-Ribose) (PAR) polymerase (PARP) inhibitors represent a promising class of novel anticancer agents. The present study explores the molecular rationale for combining veliparib (ABT-888) with camptothecin (CPT) and its…

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Nuclease Assays with pN01 from TopoGEN

Nuclease Assays with pN01 from TopoGEN

Saccharomyces cerevisiae Mre11/Rad50/Xrs2 and Ku proteins regulate association of Exo1 and Dna2 with DNA breaks Eun Yong Shim1,4, Woo-Hyun Chung2,4, Matthew L Nicolette3, Yu Zhang1, Melody Davis1, Zhu Zhu2, Tanya T Paull3, Grzegorz Ira2,* and Sang Eun Lee1,* 1Department of Molecular Medicine, Institute of Biotechnology, University of Texas Health Science Center at San Antonio, San Antonio, TX, USA, 2Department of Molecular and Human Genetics, Baylor College of Medicine, One Baylor Plaza, Houston, TX, USA and 3Department of Molecular Genetics and…

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Upcoming Holiday Closure

Upcoming Holiday Closure

TopoGEN will be closed for shipping November 24-26 for Thanksgiving. Our last official ship-day before Christmas is December 20. We will resume normal shipping operation on January 10, 2011. During this time our staff will remain available via e-mail to respond to technical questions, internet orders, and general information. Feel free to drop us a line.

Topoiosomerase Inhibition: A Study

Topoiosomerase Inhibition: A Study

Cytotoxicity and topoisomerase I/II inhibition of glycosylated 2-phenyl-indoles, 2-phenyl-benzo[b]thiophenes and 2-phenyl-benzo[b]furans Wei Shia, Sandra L. Marcusa and Todd L. Lowary Alberta Ingenuity Centre for Carbohydrate Science and Department of Chemistry, The University of Alberta, Gunning-Lemieux Chemistry Centre, Edmonton, AB T6G 2G2 Canada Abstract A panel of glycosylated DNA binding agents (1–12) designed as functional anthracycline mimics was screened against three solid-tumor cell lines (MCF-7, HT 29 and HepG2/C3A) and three non-tumor cell lines by the MTS (3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium) cell viability assay….

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DNA Unwinding Kit Controls DNA Binding Modes

DNA Unwinding Kit Controls DNA Binding Modes

Substituent control of DNA binding modes in a series of chalcogenoxanthylium photosensitizers as determined by isothermal titration calorimetry and topoisomerase I DNA unwinding assay References and further reading may be available for this article. Ruel E. McKnighta, Corresponding Author Contact Information, E-mail The Corresponding Author, Bilgehan Onogula, Shivani R. Polasania, Michael K. Gannon IIb and Michael R. Dettyb, Corresponding Author Contact Information, E-mail The Corresponding Author Department of Chemistry, State University of New York at Geneseo, 1 College Circle, Geneseo,…

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AACR 102nd Annual Meeting 2011

AACR 102nd Annual Meeting 2011

At the AACR 102nd Annual Meeting 2011, participants will present, discuss, and learn in a variety of settings: plenary lectures, symposia, minisymposia, workshops, poster sessions, and other formats. Please click the following link for Full Information on the AACR Annual Meeting in 2011

TopoGEN

TopoGEN

Our company was created by topo research active scientists who understand the needs of other researchers in the field or in areas peripheral to topoisomerases.

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