The Topoisomerase I/II in vivo Link Kit (ICE Bioassay) offers a very powerful method to deduce whether a given drug actually affects its predicted topo target in a given cancer cell in vivo. One can quantify the activity of topoisomerase enzyme in vivo with this kit.
The TopoGEN has developed a new, higher throughput ICE (In vivo Complex of Enzyme) assay that measures endogenous topoisomerase/genomic DNA covalent complexes. The one day procedure does not require a CsCl centrifugation step. The ICE Assay Kit comes with a detailed manual and is topo specific since we provide antibody that corresponds to the topo/DNA complex.