Decatenated Kinetoplast DNA Marker
Relaxed pRYG DNA Marker
Linear pRYG DNA Marker
kDNA is the ideal substrate for topoisomerase II assays because it is specific for type II reaction mechanisms. Researchers can even assay for a type II enzyme in the presence of large excess of topoisomerase I.
Supercoiled pHOT-1 DNA is a perfect substrate for assaying any topoisomerase. Specifically, the plasmid is relatively small (<3KB) which is ideal for displaying intermediate topoisomers.
Relaxed pHOT-1 DNA - ideal for assaying the supercoiling activity of DNA Gyrase.