kDNA is the ideal substrate for topoisomerase II assays because it is specific for type II reaction mechanisms. Researchers can even assay for a type II enzyme in the presence of large excess of topoisomerase I.
pRYG DNA is a supercoiled plasmid containing multiple clusters of topoisomerase II cleavage sites.
Supercoiled pHOT-1 DNA is a perfect substrate for assaying any topoisomerase. Specifically, the plasmid is relatively small (<3KB) which is ideal for displaying intermediate topoisomers.
Supercoiled pBR322 DNA is suited for assaying any topoisomerase.
Relaxed pBR322 DNA - suited for assaying supercoiling by DNA gyrase.
Decatenated Kinetoplast DNA Marker
Relaxed pRYG DNA Marker
Linear pRYG DNA Marker