kDNA is the ideal substrate for topoisomerase II assays because it is specific for type II reaction mechanisms. Researchers can even assay for a type II enzyme in the presence of large excess of topoisomerase I.
pRYG DNA is a supercoiled plasmid containing multiple clusters of topoisomerase II cleavage sites.
Supercoiled pHOT-1 DNA is a perfect substrate for assaying any topoisomerase. Specifically, the plasmid is relatively small (<3KB) which is ideal for displaying intermediate topoisomers.
Relaxed pHOT-1 DNA - ideal for assaying the supercoiling activity of DNA Gyrase.
Decatenated Kinetoplast DNA Marker
Linear pRYG DNA Marker