Homologous Recombination (HR) Reporter Cells
Human: HeLa HR Reporter Cell Line with Tet-on I-Sce1
This is a HeLa cell-based reporter kit designed to allow the customer to screen or identify agents (drugs, natural products, small molecules, synthetics, miRNAs, pathway, signaling or response genes) that affect or impact the process of HR. The kit uses GFP as an in vivo readout for the HR pathway. HR is initiated at will using Tet-on activation of a single site endonuclease. After Doxycycline induction, HR is induced in a synchronous wave in all cells that are capable of supporting the HR pathway (typically S-phase cells). The system will specifically mark cells (GFP+) that have experienced S-phase and HR has successfully been completed.
- SKU DR3001-iHRHeLa-Tet
- Size
- 25 Assays
Human: HeLa Reporter Cell Line (Includes I-Sce1 Transfection)
This is a HeLa cell-based reporter kit designed to allow the customer to screen or identify agents (drugs, natural products, small molecules, synthetics, miRNAs, pathway, signaling or response genes) that affect or impact the process of HR. The kit uses GFP as an in vivo readout for the HR pathway. HR is initiated by transfection with an I-Sce1 Expression plasmid (included). Analysis of pooled transfected cells reveals GFP+ cells in 2-3d post-transfection. GFP+ cells have successfully completed HR and can be tracked with single cell resolution.
- SKU DR3000-HRHeLa-Sce
- Size
- 25 Assays
Human: Hela HR Reporter Cell line (Includes CRISPR-CAS Transfection)
This is a cell-based reporter kit to identify agents (drugs, natural products, small molecules, synthetics, miRNAs, and genes) that affect or impact the process of HR DNA repair. The kit uses GFP as an in vivo readout for the HR pathway and RNA guided Cas9 nuclease (from S. pyogenes) to introduce a DS break. The DS DNA break sets in motion the faithful repair of GFP yielding GFP+ cells that can be used to assess the efficiency of HR. These GFP+ cells can also be followed by live imaging for single cell resolution of HR.
- SKU DR3000-HRHeLa-CPR
- Size
- 25 Assays
Human: Neuroblastoma HR Reporter Cell line (SH-SY5Y, Includes I-Sce1 Transfection)
This is a cell-based DNA Repair HR reporter that is hosted in human neuroblastoma line SH-SY5Y (or SH cells). The kit is designed to allow the customer to screen or identify agents (drugs, natural products, small molecules, synthetics, miRNAs, pathway, signaling or response genes) that affect or impact the process of HR. The kit uses GFP as an in vivo readout for the HR pathway in a neuro-derived line that can be differentiated. HR is initiated by transfection with an I-Sce1 Expression plasmid (included). Analysis of pooled transfected cells reveals GFP+ cells in 2-3d post-transfection. GFP+ cells have successfully completed HR and can be tracked with single cell resolution.
- SKU DR3000-HRSH-Sce
- Size
- 25 Assays
Mouse: 3T3 HR Reporter Cell Line (Includes I-Sce1 Transfection)
This is a mouse 3T3 cell-based reporter kit designed to allow the customer to screen or identify agents (drugs, natural products, small molecules, synthetics, miRNAs, pathway, signaling or response genes) that affect or impact the process of HR. The kit uses GFP as an in vivo readout for the HR pathway. These cells are easily synchronized and provide the opportunity to analyze HR in the cell cycle.
- SKU DR3000-HR3T3-Sce
- Size
- 25 Assays
Mouse: Neuronal Lineage HR Reporter Cell line (Includes I-Sce1 Transfection)
This is a cell-based reporter kit designed to study homologous recombination or HR repair in a mouse neuronal cell line (CN1.4). The cell line expresses numerous neuronal markers, including neurofilaments, glutamic acid decarboxylase 67, neuron-specific enolase, and the BG21 isoform of the myelin basic protein gene. These cells host a reporter gene (GFP) as an in vivo readout for the HR pathway. The cells can be terminally differentiated in vitro.
- SKU DR3000-HRCN1.4-Sce
- Size
- 25 Assays
Custom Cell Based Screening Kit for (HR) Pathway
Custom cell based screening kit for homologous repair (HR) pathway. This kit allows customer to create a cell line host for HR.
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A new method to quantify endogenous Topoisomerase action in E. coli cells is nicely described in this paper. The technique is based on the In Vivo Link Kit, also known as the ICE bioassay. Article Source: American Society for Mi...