TopoGEN provides this reagent as a control poison/inhibitor of topoisomerase II. For in vitro cleavage detection, we recommend that the drug be tested over a wide range of uM concentrations; however, cleavages are strongly influenced by the amount of topoisomerase in the reaction as well as the type of assay being employed (SDSK+ vs. Radioactive End Labeled DNA vs. testing for plasmid cleavages). Note that large amounts of enzyme will be required to detect cleavage complex, compared to assays that measure catalytic activity.
Chemical Name: 9-[(4,6-O-Ethylidene-β-D-glucopyranosyl)oxy]-5,8,- 8a,9-tetrahydro-5-(4-hydroxy-3,5-dimethoxyphenyl)- furo[3',4':6,7] naphtho[2,3-d]-1,3-dioxol-6(5aH)-one
Molecular Formula: C29H32O13
Molecular Weight: 588.56
TG4140 contains lyophilized Etoposide. Addition of 0.25 ml of solvent (DMSO) to drug will yield a working stock of 10mM. The drug is pre-tested and shown to induce topo cleavages using purified human topoisomerase from TopoGEN.
The dry product is shipped at ambient temperature. Store rehydrated at -20°C.