Kit Replenishments

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Buffers and reagents for use in TopoGEN kits.

Topoisomerase I Assay Buffer

10X Topoisomerase I Assay Buffer (TGS).  The buffer is optimized and tested for relaxation and cleavages by topo I.

 
Topoisomerase I Assay Buffer
$75.00

E. coli DNA Gyrase Assay Buffer

E. coli DNA gyrase assay buffer supplied as a 5X stock solution.  The buffer is optimized and tested for E. coli DNA gyrase only. 

 
E. coli DNA Gyrase Assay Buffer
$75.00

Topoisomerase IV Assay Buffer

5x Topoisomerase IV Assay Buffer is supplied in two parts that must be mixed prior to use.  The complete Topo IV Assay Buffer (A+B) is optimized and tested for decatenation and relaxation activity of E. coli topo IV.

 
Topoisomerase IV Assay Buffer
$95.00
  • SKU TG4032
  • Size
  • 1 ml (A), 100 ul (B)

5X Universal Stop Buffer

5X Stop Buffer used to terminate topoisomerase reactions.

 
5X Universal Stop Buffer
$85.00

10X Gel Loading Buffer

Agarose Gel Loading Buffer (10X)

 
10X Gel Loading Buffer
$85.00

Proteinase K

10X Proteinase K is a subtilisin-like endolytic protease that is isolated from the saprophytic fungus Tritirachium album.  It has a high activity that is stable across a wide range of pH and temperature conditions and is suited to short digestion times. 

 
Proteinase K
$65.00

Topoisomerase II Assay Buffer

Topoisomerase II assay buffer is supplied as a 10X stock solution in two parts (A+B) that must be mixed prior to use.  The 5X Complete Topo II Assay Buffer is optimized and tested for decatenation and relaxation activity of eukaryotic topo II.

 
Topoisomerase II Assay Buffer
$95.00
  • SKU TG4040
  • Size
  • 1 ml (A), 1 ml (B)

10% Sodium Dodecyl Sulfate

High purity SDS is dissolved in triple glass distilled/deionized, sterile water to 10% (w/v).

 
10% Sodium Dodecyl Sulfate
$65.00

20% Sarkosyl

20% Sarkosyl

 
20% Sarkosyl
$95.00

Topoisomerase II Dilution Buffer

Topo II Dilution Buffer (1X).  Optimized and tested for use as a diluent for TopoGEN's type II topoisomerase.

 
Topoisomerase II Dilution Buffer
$85.00

Topoisomerase I Dilution Buffer

Topo I Dilution Buffer (1X).  Optimized and tested for use as a diluent for recombinant human topo I enzyme from TopoGEN.

 
Topoisomerase I Dilution Buffer
$85.00

20mM ATP

20mM ATP

 
20mM ATP
$85.00

S. Aureus Gyrase Assay Buffer

S. Aureus Gyrase Assay Buffer in aqueous solution

 
S. Aureus Gyrase Assay Buffer
$85.00

Potassium Glutamate

5X Potassium Glutamate (1ml). 

 
Potassium Glutamate
$85.00