10X Topoisomerase I Assay Buffer (TGS). The buffer is optimized and tested for relaxation and cleavages by topo I.
E. coli DNA gyrase assay buffer supplied as a 5X stock solution. The buffer is optimized and tested for E. coli DNA gyrase only.
5x Topoisomerase IV Assay Buffer is supplied in two parts that must be mixed prior to use. The complete Topo IV Assay Buffer (A+B) is optimized and tested for decatenation and relaxation activity of E. coli topo IV.
5X Stop Buffer used to terminate topoisomerase reactions.
Agarose Gel Loading Buffer (10X)
10X Proteinase K is a subtilisin-like endolytic protease that is isolated from the saprophytic fungus Tritirachium album. It has a high activity that is stable across a wide range of pH and temperature conditions and is suited to short digestion times.
Topoisomerase II assay buffer is supplied as a 10X stock solution in two parts (A+B) that must be mixed prior to use. The 5X Complete Topo II Assay Buffer is optimized and tested for decatenation and relaxation activity of eukaryotic topo II.
High purity SDS is dissolved in triple glass distilled/deionized, sterile water to 10% (w/v).
Topo II Dilution Buffer (1X). Optimized and tested for use as a diluent for TopoGEN's type II topoisomerase.
Topo I Dilution Buffer (1X). Optimized and tested for use as a diluent for recombinant human topo I enzyme from TopoGEN.
S. Aureus Gyrase Assay Buffer in aqueous solution