Supercoiled pBR322 DNA is suited for assaying any topoisomerase.
Relaxed pBR322 DNA - suited for assaying supercoiling by DNA gyrase.
This is a HeLa cell-based reporter kit designed to allow the customer to screen or identify agents (drugs, natural products, small molecules, synthetics, miRNAs, pathway, signaling or response genes) that affect or impact the process of HR. The kit uses GFP as an in vivo readout for the HR pathway. HR is initiated at will using Tet-on activation of a single site endonuclease. After Doxycycline induction, HR is induced in a synchronous wave in all cells that are capable of supporting the HR pathway (typically S-phase cells). The system will specifically mark cells (GFP+) that have experienced S-phase and HR has successfully been completed.
This is a HeLa cell-based reporter kit to analyze NHEJ in live cells. Specifically this kit will identify agents (drugs, natural products, small molecules, synthetics, miRNAs, and other genes) that affect or impact NHEJ repair. The kit uses GFP as an in situ readout for NHEJ activity in a cell context. The NHEJ process is activated with high penetrance and synchrony using Doxycycline to induce an endonuclease that specifically cuts the GFP reporter, thereby activating NHEJ. A specific reporter based assay for NHEJ can be very beneficial for anti-cancer drug discovery projects, learning more about the process of NHEJ repair and establishing intersecting pathways, druggable pathway targets, aging and disease modeling in cells.