Topo Assay Kits, Drug Screening, and ICE Assay Kits
Topoisomerase In Vivo Link Kit
The Topoisomerase I/II in vivo Link Kit (ICE Bioassay) offers a very powerful method to deduce whether a given drug actually affects its predicted topo target in a given cancer cell in vivo. One can quantify the activity of topoisomerase enzyme in vivo with this kit.
Topoisomerase I Assay Buffer
10X Topoisomerase I Assay Buffer (TGS). The buffer is optimized and tested for relaxation and cleavages by topo I.
E. coli DNA Gyrase Assay Buffer
E. coli DNA gyrase assay buffer supplied as a 5X stock solution. The buffer is optimized and tested for E. coli DNA gyrase only.
Topoisomerase IV Assay Buffer
5x Topoisomerase IV Assay Buffer is supplied in two parts that must be mixed prior to use. The complete Topo IV Assay Buffer (A+B) is optimized and tested for decatenation and relaxation activity of E. coli topo IV.
Proteinase K
10X Proteinase K is a subtilisin-like endolytic protease that is isolated from the saprophytic fungus Tritirachium album. It has a high activity that is stable across a wide range of pH and temperature conditions and is suited to short digestion times.
Topoisomerase II Assay Buffer
Topoisomerase II assay buffer is supplied as a 10X stock solution in two parts (A+B) that must be mixed prior to use. The 5X Complete Topo II Assay Buffer is optimized and tested for decatenation and relaxation activity of eukaryotic topo II.
10% Sodium Dodecyl Sulfate
High purity SDS is dissolved in triple glass distilled/deionized, sterile water to 10% (w/v).
Topoisomerase II Dilution Buffer
Topo II Dilution Buffer (1X). Optimized and tested for use as a diluent for TopoGEN's type II topoisomerase.
Topoisomerase I Dilution Buffer
Topo I Dilution Buffer (1X). Optimized and tested for use as a diluent for recombinant human topo I enzyme from TopoGEN.
Human Topoisomerase ICE Assay Kit
The TopoGEN has developed a new, higher throughput ICE (In vivo Complex of Enzyme) assay that measures endogenous topoisomerase/genomic DNA covalent complexes. The one day procedure does not require a CsCl centrifugation step. The ICE Assay Kit comes with a detailed manual and is topo specific since we provide antibody that corresponds to the topo/DNA complex.
Topoisomerase I Assay Kit
The Topoiosomerase I Assay Kit is a specific assay for topo I in a crude mixture of heterologous proteins. It is generally easy to interpret an assay that quantifies topo I activity.
Topoisomerase I Drug Screening Kit
The Topoisomerase I Drug Screening Kit allows the researcher to model drug action with novel topo I active agents. For example, is it a poison or an inhibitor?
Human Topoisomerase II Assay Kit
The Human Topoisomerase II Assay Kit is designed for researchers who wish to measure topo II activity in a crude extract. It is highly specific and can be quantified.
Topoisomerase II Drug Screening Kit
The Topoisomerase II Drug Screening Kit will help you interpret how a novel drug affects topo II action.
Gyrase Assay Kit
The DNA Gyrase Assay Kit will allow one to quantify gyrase activity in a crude mix of other proteins (other topoisomerase enzymes for example). It is ideal for characterizing inhibitors as well.
DNA Intercalator/Unwinding Kit
The DNA Intercalator/Unwinding Kit wil reveal how a particular drug intercalates and unwinds the DNA helix.
Topoisomerase II Drug Screening Kit (kDNA based)
This kDNA-based Topo II Drug Kit is designed to idendify agents that target type II topoisomerases acting as catalytic inhibitors, primarily; however, poisons can also be detected.
- SKU TG1019-1A
- Size
- 100 Assays + 100 Units Enzyme
- SKU TG1019-2A
- Size
- 250 Assays + 250 Units Enzyme
- SKU TG1019-3A
- Size
- 250 Assays + 500 Units Enzyme
E. coli Topoisomerase IV Drug Screening Kit
The Topoisomerase IV Drug Screeing Kit is designed for researchers who wish to measure the realease of topoisomerase from covalent complexes that form as a result of drug treatment. It is highly specific and can be quantified.
- SKU TG1007-1A
- Size
- 100 Assays + 100 Units Enzyme
- SKU TG1007-2A
- Size
- 250 Assays + 250 Units Enzyme
- SKU TG1007-3A
- Size
- 250 Assays + 500 Units Enzyme
E. coli DNA Gyrase and Relaxed DNA Assay Kit
TopoGEN sells E. coli DNA gyrase along with the relaxed DNA subtrate (pHOT-1). The enzyme is very active and can be purchased in large quantities for screening studies.
E. coli DNA Gyrase Drug Screening Kit
DNA Gyrase is an important target for anti-bacterial drugs and new antibiotics are constantly required to deal with drug resistant strains of pathogens. This Kit is designed to identify agents (small molecules, natural products, biological material or synthetics) that directly or indirectly target DNA Gyrase from E. coli. This Kit includes homogenously purified DNA Gyrase.
TopI-NucEx Kit
Prepare a nuclear extract enriched for type I topoisomerase (topo I) activity and intact 100 kDa polypeptide.
TopII-NucEx Kit
Prepare a nuclear extract enriched for type II topoisomerase (topo II) activity and topo II polypeptide.
Topoisomerase I Hi-Loading Inhibition Kit
This kit is designed to allow the customer to screen for agents (drugs, natural products, small molecules, synthetics) that block the catalytic activity of human topoisomerase I (topo I).
SDS-K+ Precipitation Kit (in vitro)
Designed for researchers who wish to monitor topoisomerase I or II covalent binding to a DNA target in vitro AND in vivo.
SDS-K+ Precipitation Kit (in vivo)
Designed for researchers who wish to monitor topoisomerase I or II covalent binding to a DNA target in vitro AND in vivo.
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TopoGEN, Inc.
27960 County Rd. 319
Hangar B
Buena Vista, CO 81211
USA - 614-451-5810
- 614-559-3932
- Mon-Fri: 8AM to 5PM
Closed Saturday and Sunday 
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The authors have used a clever genetic screen to identify “Cheater mutations” in genes that confer the ability of cells to behave in a non-cooperative fashion in a tissue context. These cheater cells could for example, outgrow sur...