Topo Assay Kits, Drug Screening, and ICE Assay Kits

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Topoisomerase Assay Kits, Drug Screening Kits, New ICE Assay Kits, Nuclear Extraction Kits, and replenishments.

Topoisomerase I Assay Kit

The Topoiosomerase I Assay Kit is a specific assay for topo I in a crude mixture of heterologous proteins. It is generally easy to interpret an assay that quantifies topo I activity.

 
Topoisomerase I Assay Kit
$195.00
Topoisomerase I Assay Kit
$295.00

Gyrase Assay Kit

The DNA Gyrase Assay Kit will allow one to quantify gyrase activity in a crude mix of other proteins (other topoisomerase enzymes for example). It is ideal for characterizing inhibitors as well.

 
Gyrase Assay Kit
$195.00

Human Topoisomerase II Assay Kit

The  Human Topoisomerase II Assay Kit is designed for researchers who wish to measure topo II activity in a crude extract. It is highly specific and can be quantified.

 
Human Topoisomerase II Assay Kit
$195.00
Human Topoisomerase II Assay Kit
$295.00

SDS-K+ Precipitation Kit (in vivo)

Designed for researchers who wish to monitor topoisomerase I or II covalent binding to a DNA target in vitro AND in vivo.

 
SDS-K+ Precipitation Kit (in vivo)
$195.00
SDS-K+ Precipitation Kit (in vivo)
$295.00

Topoisomerase I Drug Screening Kit

The Topoisomerase I Drug Screening Kit allows the researcher to model drug action with novel topo I active agents. For example, is it a poison or an inhibitor?

 
Topoisomerase I Drug Screening Kit
$275.00
Topoisomerase I Drug Screening Kit
$375.00

Topoisomerase In Vivo Link Kit

The Topoisomerase I/II in vivo Link Kit (ICE Bioassay) offers a very powerful method to deduce whether a given drug actually affects its predicted topo target in a given cancer cell in vivo. One can quantify the activity of topoisomerase enzyme in vivo with this kit.

 
Topoisomerase In Vivo Link Kit
$395.00
Topoisomerase In Vivo Link Kit
$395.00

Topoisomerase II In Vivo Link Kit

The Topoisomerase II in vivo Link Kit provides a very powerful method to deduce whether a given drug actually affects its predicted topo target in a given cancer cell in vivo. One can quantify the activity of topoisomerase in vivo with this kit.

 
Topoisomerase II In Vivo Link Kit
$395.00

E. coli Topoisomerase IV Drug Screening Kit

The Topoisomerase IV Drug Screeing Kit is designed for researchers who wish to measure the realease of topoisomerase from covalent complexes that form as a result of drug treatment.  It is highly specific and can be quantified.

 
E. coli Topoisomerase IV Drug Screening Kit
$275.00
E. coli Topoisomerase IV Drug Screening Kit
$375.00

Topoisomerase II Drug Screening Kit

The Topoisomerase II Drug Screening Kit will help you interpret how a novel drug affects topo II action.

 
Topoisomerase II Drug Screening Kit
$275.00
Topoisomerase II Drug Screening Kit
$375.00

SDS-K+ Precipitation Kit (in vitro)

Designed for researchers who wish to monitor topoisomerase I or II covalent binding to a DNA target in vitro AND in vivo. 

 
SDS-K+ Precipitation Kit (in vitro)
$315.00
SDS-K+ Precipitation Kit (in vitro)
$415.00

DNA Intercalator/Unwinding Kit

The DNA Intercalator/Unwinding Kit wil reveal how a particular drug intercalates and unwinds the DNA helix.  

 
DNA Intercalator/Unwinding Kit
$395.00
  • SKU TG1025
  • Size
  • 50 Experiments

E. coli DNA Gyrase and Relaxed DNA Assay Kit

TopoGEN sells E. coli DNA gyrase along with the relaxed DNA subtrate (pHOT-1).  The enzyme is very active and can be purchased in large quantities for screening studies.

E. coli DNA Gyrase and Relaxed DNA Assay Kit
$235.00
E. coli DNA Gyrase and Relaxed DNA Assay Kit
$755.00
E. coli DNA Gyrase and Relaxed DNA Assay Kit
$1,195.00
E. coli DNA Gyrase and Relaxed DNA Assay Kit
$2,195.00

Topoisomerase I Assay Buffer

10X Topoisomerase I Assay Buffer (TGS).  The buffer is optimized and tested for relaxation and cleavages by topo I.

 
Topoisomerase I Assay Buffer
$75.00

E. coli DNA Gyrase Assay Buffer

E. coli DNA gyrase assay buffer supplied as a 5X stock solution.  The buffer is optimized and tested for E. coli DNA gyrase only. 

 
E. coli DNA Gyrase Assay Buffer
$75.00

Topoisomerase IV Assay Buffer

5x Topoisomerase IV Assay Buffer is supplied in two parts that must be mixed prior to use.  The complete Topo IV Assay Buffer (A+B) is optimized and tested for decatenation and relaxation activity of E. coli topo IV.

 
Topoisomerase IV Assay Buffer
$95.00
  • SKU TG4032
  • Size
  • 1 ml (A), 100 ul (B)

5X Universal Stop Buffer

5X Stop Buffer used to terminate topoisomerase reactions.

 
5X Universal Stop Buffer
$85.00

10X Gel Loading Buffer

Agarose Gel Loading Buffer (10X)

 
10X Gel Loading Buffer
$85.00

Proteinase K

10X Proteinase K is a subtilisin-like endolytic protease that is isolated from the saprophytic fungus Tritirachium album.  It has a high activity that is stable across a wide range of pH and temperature conditions and is suited to short digestion times. 

 
Proteinase K
$65.00

Topoisomerase II Assay Buffer

Topoisomerase II assay buffer is supplied as a 10X stock solution in two parts (A+B) that must be mixed prior to use.  The 5X Complete Topo II Assay Buffer is optimized and tested for decatenation and relaxation activity of eukaryotic topo II.

 
Topoisomerase II Assay Buffer
$95.00
  • SKU TG4040
  • Size
  • 1 ml (A), 1 ml (B)

10% Sodium Dodecyl Sulfate

High purity SDS is dissolved in triple glass distilled/deionized, sterile water to 10% (w/v).

 
10% Sodium Dodecyl Sulfate
$65.00

20% Sarkosyl

20% Sarkosyl

 
20% Sarkosyl
$95.00

Topoisomerase II Dilution Buffer

Topo II Dilution Buffer (1X).  Optimized and tested for use as a diluent for TopoGEN's type II topoisomerase.

 
Topoisomerase II Dilution Buffer
$85.00

Topoisomerase I Dilution Buffer

Topo I Dilution Buffer (1X).  Optimized and tested for use as a diluent for recombinant human topo I enzyme from TopoGEN.

 
Topoisomerase I Dilution Buffer
$85.00

Topoisomerase II Drug Screening Kit (kDNA based)

This kDNA-based Topo II Drug Kit is designed to idendify agents that target type II topoisomerases acting as catalytic inhibitors, primarily; however, poisons can also be detected. 

 
Topoisomerase II Drug Screening Kit (kDNA based)
$275.00
Topoisomerase II Drug Screening Kit (kDNA based)
$375.00

20mM ATP

20mM ATP

 
20mM ATP
$85.00

S. Aureus Gyrase Assay Buffer

S. Aureus Gyrase Assay Buffer in aqueous solution

 
S. Aureus Gyrase Assay Buffer
$85.00

Potassium Glutamate

5X Potassium Glutamate (1ml). 

 
Potassium Glutamate
$85.00

TopII-NucEx Kit

Prepare a nuclear extract enriched for type II topoisomerase (topo II) activity and topo II polypeptide. 

 
TopII-NucEx Kit
$275.00

TopI-NucEx Kit

Prepare a nuclear extract enriched for type I topoisomerase (topo I) activity and intact 100 kDa polypeptide.

 
TopI-NucEx Kit
$275.00

E. coli DNA Gyrase Drug Screening Kit

DNA Gyrase is an important target for anti-bacterial drugs and new antibiotics are constantly required to deal with drug resistant strains of pathogens. This Kit is designed to identify agents (small molecules, natural products, biological material or synthetics) that directly or indirectly target DNA Gyrase from E. coli.  This Kit includes homogenously purified DNA Gyrase.

E. coli DNA Gyrase Drug Screening Kit
$295.00
E. coli DNA Gyrase Drug Screening Kit
$795.00
E. coli DNA Gyrase Drug Screening Kit
$1,255.00
E. coli DNA Gyrase Drug Screening Kit
$2,255.00

Topoisomerase I Hi-Loading Inhibition Kit

This kit is designed to allow the customer to screen for agents (drugs, natural products, small molecules, synthetics) that block the catalytic activity of human topoisomerase I (topo I).

 
Topoisomerase I Hi-Loading Inhibition Kit
$325.00
Topoisomerase I Hi-Loading Inhibition Kit
$395.00

Human Topoisomerase ICE Assay Kit

The  TopoGEN has developed a new, higher throughput ICE (In vivo Complex of Enzyme) assay that measures endogenous topoisomerase/genomic DNA covalent complexes. The one day procedure does not require a CsCl centrifugation step. The ICE Assay Kit comes with a detailed manual and is topo specific since we provide antibody that corresponds to the topo/DNA complex.

Human Topoisomerase ICE Assay Kit
$245.00
Human Topoisomerase ICE Assay Kit
$345.00
Human Topoisomerase ICE Assay Kit
$345.00
Human Topoisomerase ICE Assay Kit
$345.00
Human Topoisomerase ICE Assay Kit
$345.00
Human Topoisomerase ICE Assay Kit
$345.00