|Topoisomerase I Assay Kit|
|TG1015-1||100 Assays||$195.00||Order Now|
|TG1015-2||250 Assays||$295.00||Order Now|
Topoisomerase I Assay Kit Product Description
This kit contains reagents for routine detection of type I topoisomerase activity. DNA markers (included) allow unequivocal detection of the enzyme. The assay is specific for topoisomerase I; (eukaryotic type II topoisomerases require MgCl2 and ATP). The standard topo I assay kit is supplied with EDTA containing reaction buffer (minus Mg++) in order to prevent nucleolytic nicking of the supercoiled substrate (although we note that some eukaryotic type I activities are stimulated by Mg++). This assay will work even with crude extracts. A detailed manual of instruction (see link above) is included that describes how to run the gels to maximally resolve topoisomers for analysis of DNA unwinding.
Topoisomerase I Assay Kit Contents (representative for 100 assay kit size):
-Supercoiled pHOT-1 DNA (25 µg in 100 µl TE buffer, or 0.25 µg/ul)
-Relaxed pHOT-1 DNA marker, relaxed by topo I (50 ul at 0.05 µg/ml in gel loading buffer)
-10X Topo I assay buffer (300 µl)
-5X Stop buffer/gel loading dye (600 µl)
-Detailed instruction manual
-Sample data and documentation of all controls
Reviews and Citations:
The kit may be shipped at ambient temperature or on ice (dry ice or wet ice). The DNAs should be stored at 4°C and the buffers stored at -20°C upon receipt. Avoid frequent freeze/thaw cycles with the plasmid as this may contribute to DNA breakage.