Human Topoisomerase I Assay Kit | ||||
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SKU | Size | Price | Qty | |
TG1015-1 | 100 Assays | $255.00 | Add to Cart | |
TG1015-2 | 250 Assays | $345.00 | Add to Cart | |
TG1015-1A | 100 Assays + 100 Units Enzyme | $355.00 | Add to Cart | |
TG1015-2A | 250 Assays + 250 Units Enzyme | $545.00 | Add to Cart | |
TG1015-3A | 500 Assays + 500 Units Enzyme | $645.00 | Add to Cart |
Topoisomerase I Assay Kit Product Description
This kit contains reagents for routine detection of Top1 activity. DNA markers (included) allow unequivocal detection of the enzyme. The assay is specific for topoisomerase I; (eukaryotic type II topoisomerases require MgCl2 and ATP). The standard topo I assay kit is supplied with EDTA containing reaction buffer (minus Mg++) in order to prevent nucleolytic nicking of the supercoiled substrate (although we note that some eukaryotic type I activities are stimulated by Mg++). This assay will work even with crude extracts. A detailed manual of instruction (see link above) is included that describes how to run the gels to maximally resolve topoisomers for analysis of DNA unwinding.
Topoisomerase I Assay Kit Contents (representative for 100 assay kit size):
-100 Units Human Top1 Enzyme (available with catalog number TG1015-1A only)
-Supercoiled pHOT-1 DNA (25 µg in 100 µl TE buffer, or 0.25 µg/ul)
-Relaxed pHOT-1 DNA marker, relaxed by topo I (50 ul at 0.05 µg/ml in gel loading buffer)
-10X Topo I assay buffer (300 µl)
-5X Stop buffer/gel loading dye (600 µl)
-Detailed instruction manual
-Sample data and documentation of all controls
Reviews and Citations:
This kit is shipped at ambient temperature or on dry ice if purchased with enzyme. Store enzyme at -70° C. All other components may be stored at 4° C. Avoid frequent freeze/thaw cycles with the plasmid as this may contribute to DNA breakage.