|E. coli Bacterial DNA Gyrase|
|TG2000G-1||100 Units||$215.00||Add to Cart|
|TG2000G-3||500 Units||$505.00||Add to Cart|
|TG2000G-5||1000 Units||$925.00||Add to Cart|
|TG2000G-7||2000 Units||$1,715.00||Add to Cart|
E. coli Bacterial DNA Gyrase Product Description
Highly purified DNA Gyrase is offered as a holoenzyme (contains both A,B subunits). This is an excellent reagent for supercoiling plasmids in vitro or for novel drug screens.
Quality Control Tests:
Nuclease contamination: assayed by testing for linear KDNA and linear plasmid DNA formation. Incubation of 1 µg of catenated KDNA or supercoiled pUC19 DNA for 4 hrs. at 37°C (under gyrase assay conditions and with or without ATP).
Supercoiling assays are carried out using relaxed pBR-322 DNA under conditions specified in the protocol sheets provided with the product. We also perform assays using kDNA as substrate. For details on this decatenation/supercoiling assay please refer to our technical literature (link provided above). One unit of gyrase activity will supercoil 0.2 ug of plasmid in 30 min at 37°C.
A 5X assay uffer and dilution buffer is included with the enzyme. These are also sold separately as 5X Gyrase Assay Buffer (TG4030) Gyrase Dilution Buffer (please enquire).
Purification of Gyrase A and B Subunits:
Reviews and Citations
The enzyme is shipped on dry ice and should be stored at -70°C. We also recommend that the enzyme be aliquoted after the first thaw (repeated rounds of freeze/thaw may cause loss of activity); the enzyme activity is stable for 1-3 days on ice.